Evaluating the toxicity of silicon dioxide nanoparticles on neural stem cells using rna-seq
文献类型：期刊论文
| 作者 | Sun, Dayu1,2,3; Gong, Linji4,5; Xie, Jing1,3; He, Xiao1,3; Chen, Siyu1,3; Luodan, A.1,3; Li, Qiyou1,3; Gu, Zhanjun4,5; Xu, Haiwei1,3 |
| 刊名 | Rsc advances
 |
| 出版日期 | 2017 |
| 卷号 | 7期号:75页码:47552-47564 |
| ISSN号 | 2046-2069 |
| DOI | 10.1039/c7ra09512k |
| 通讯作者 | Gu, zhanjun(zjgu@ihep.ac.cn) ; Xu, haiwei(haiweixu2001@163.com) |
| 英文摘要 | Neural stem cells are characterized by self-renewal and multipotency, and a capacity to regenerate in response to brain injury or neurodegenerative disease. silicon dioxide nanoparticles (sio2 nps) are novel materials, which enable the delivery of specific payloads to stem cells; for example, genes or proteins, to enable cell-fate manipulation, or tracer materials, to enable in vivo tracing. however, little is known about the dose-dependent cytotoxicity of sio2 nps, and how exposure to sio2 nps changes mrna expression profiles in neural stem cells. in this study, a mouse c17.2 neural stem cell line was treated with 90 nm monodisperse fluorescein isothiocyanate-sio2 nps at 0, 100, 200 and 400 mg ml(-1) for 48 hours. internalization of sio2 nps was observed in c17.2 cells in a dose-dependent manner. sio2 np exposure induced apoptosis and inhibited cell proliferation in the c17.2 cell line at dosage levels of 200 mg ml(-1) and above. microscopically, mitochondrial swelling and cristae fracture were observed. furthermore, next generation rna sequencing (rna-seq) indicated that high-dose sio2 np exposure specifically inhibited transcription of glutathione-s-transferase (gst) genes, including gstm1, gstm7 and gstt1. these results suggest that application of high-dose sio2 nps to the nervous system may cause neurotoxicity, induce apoptosis and reduce neural stem cell proliferation by inhibiting gst gene expression. |
| WOS关键词 | BLOOD-BRAIN-BARRIER ; DRUG-DELIVERY ; IN-VIVO ; NEURODEGENERATIVE DISEASES ; OXIDE NANOPARTICLES ; EPITHELIAL-CELLS ; GENE DELIVERY ; PROLIFERATION ; EXPRESSION ; REVEALS |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000412977000039 |
| 出版者 | ROYAL SOC CHEMISTRY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2177124 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Gu, Zhanjun; Xu, Haiwei |
| 作者单位 | 1.Third Mil Med Univ, Southwest Hosp, Southwest Eye Hosp, Chongqing 400038, Peoples R China 2.Third Mil Med Univ, Dept Physiol, Chongqing 400038, Peoples R China 3.Key Lab Visual Damage & Regenerat & Restorat Chon, Chongqing 400038, Peoples R China 4.Chinese Acad Sci, Inst High Energy Phys, Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Dayu,Gong, Linji,Xie, Jing,et al. Evaluating the toxicity of silicon dioxide nanoparticles on neural stem cells using rna-seq[J]. Rsc advances,2017,7(75):47552-47564. |
| APA | Sun, Dayu.,Gong, Linji.,Xie, Jing.,He, Xiao.,Chen, Siyu.,...&Xu, Haiwei.(2017).Evaluating the toxicity of silicon dioxide nanoparticles on neural stem cells using rna-seq.Rsc advances,7(75),47552-47564. |
| MLA | Sun, Dayu,et al."Evaluating the toxicity of silicon dioxide nanoparticles on neural stem cells using rna-seq".Rsc advances 7.75(2017):47552-47564. |
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来源：高能物理研究所
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