Thermal unfolding process of lysozyme on pegylated gold nanoparticles reveals length-dependent effects of peg layer
文献类型：期刊论文
| 作者 | Cai, Chengxu1,2; Wang, Mingzhe1,2; Wang, Liming1; Wang, Bing3; Feng, Weiyue3; Ghen, Chunying1 |
| 刊名 | Journal of nanoscience and nanotechnology
 |
| 出版日期 | 2018-08-01 |
| 卷号 | 18期号:8页码:5542-5550 |
| 关键词 | Gold nanoparticles Pegylation Lysozyme Synchrotron radiation circular dichroism |
| ISSN号 | 1533-4880 |
| DOI | 10.1166/jnn.2018.15416 |
| 通讯作者 | Feng, weiyue() ; Ghen, chunying() |
| 英文摘要 | Characterization of bio-nano interface is crucial for developing safer and more efficient nanoparticles in nanomedical application. pegylation is commonly used in nanocarrier for drug delivery, as it confers nanoparticles good stability, stealth effect and better targeting specificity compared with those without pegylation. however, the protein binding state on pegylated aunp is still limited known. in present work, we prepared 13 nm aunps and then pegylated them with thiol peg methoxy. lysozyme is selected as a model protein and to investigate the interactions on protein-pegylated/aunp interface. the thermal unfolding processes of lysozyme in absence and presence of pegylated aunp were measured by synchrotron radiation based circular dichroism (srcd), dynamic light scattering (dls) and infrared spectroscopy (ir). the results suggest that in terms of secondary structural changes, alpha helix content is decreased, while beta sheet content is increased, and thus the adsorbed lysozyme may be present in peg layer. |
| WOS关键词 | TRANSFORM-INFRARED-SPECTROSCOPY ; CIRCULAR-DICHROISM SPECTROSCOPY ; SECONDARY STRUCTURE ANALYSES ; PROTEIN ADSORPTION ; CONFORMATIONAL-CHANGES ; THERMODYNAMICS ; SURFACES |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| WOS类目 | Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter |
| 语种 | 英语 |
| WOS记录号 | WOS:000426059800042 |
| 出版者 | AMER SCIENTIFIC PUBLISHERS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2178037 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Feng, Weiyue; Ghen, Chunying |
| 作者单位 | 1.Natl Ctr Nanosci & Technol, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Chengxu,Wang, Mingzhe,Wang, Liming,et al. Thermal unfolding process of lysozyme on pegylated gold nanoparticles reveals length-dependent effects of peg layer[J]. Journal of nanoscience and nanotechnology,2018,18(8):5542-5550. |
| APA | Cai, Chengxu,Wang, Mingzhe,Wang, Liming,Wang, Bing,Feng, Weiyue,&Ghen, Chunying.(2018).Thermal unfolding process of lysozyme on pegylated gold nanoparticles reveals length-dependent effects of peg layer.Journal of nanoscience and nanotechnology,18(8),5542-5550. |
| MLA | Cai, Chengxu,et al."Thermal unfolding process of lysozyme on pegylated gold nanoparticles reveals length-dependent effects of peg layer".Journal of nanoscience and nanotechnology 18.8(2018):5542-5550. |
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来源：高能物理研究所
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