3D-printed pneumatic microfluidic mixer for colorimetric detection of Listeria monocytogenes
文献类型：会议论文
| 作者 | Tung, Steve1,3; Li, Yanbin2,4; Harris, Nathan3; Yao P(姚萍)2,3 ; Wang JY(王敬依)1; Xi, Xinge4; Wang, Ronghui4
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| 出版日期 | 2018 |
| 会议日期 | July 29 - August 1, 2018 |
| 会议地点 | Detroit, MI, United states |
| 关键词 | 3D printing microfluidic mixer Listeria monocytogenes rapid detection |
| 页码 | 1-10 |
| 英文摘要 | 3D printing can significantly improve the current fabrication techniques for microfluidic devices due to its ability to create truly 3D structures in a single step. In this study, an active pneumatic microfluidic mixer was designed and fabricated using an extrusion-based 3D printer for rapid detection of Listeria monocytogenes. The printed material of the mixer is flexible, semi-transparent and inexpensive. The fabrication time is significantly shorter than the traditional micromolding process. The printed mixer consists of two pneumatic air chambers and one mixing chamber designed for a fluidic sample size of 100 μL. The length, width, and height of the mixer chip are 13, 12.7, and 9 mm, respectively. The performance of the mixer was tested for different actuation frequencies and pneumatic pressures. The completed 3D-printed mixer was successfully applied to the colorimetric detection of L. monocytogenes for a concentration range from 102 to 108 cfu/mL using an enzyme-linked immunosorbent assay. The experimental results showed the microfluidic mixer could enhance the mixing efficiency of the fluidic sample through pneumatically actuated diaphragms. In addition, the mixer could accelerate the color development caused by target L. monocytogenes, and the observed color changes could be discriminated within 5 minutes by naked eye. The present work will contribute to the development and optimization of a prototype for rapid detection of L. monocytogenes in food samples. It provides an effective technical approach to realize the fabrication of low-cost microfluidic chips for efficient reagent mixing in microscale biochemical detection systems. |
| 源文献作者 | American Society of Agricultural and Biological Engineers (ASABE) |
| 产权排序 | 4 |
| 会议录 | ASABE 2018 Annual International Meeting
 |
| 会议录出版者 | American Society of Agricultural and Biological Engineers |
| 会议录出版地 | St. Joseph |
| 语种 | 英语 |
| 源URL | [http://ir.sia.cn/handle/173321/23360]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 通讯作者 | Yao P(姚萍) |
| 作者单位 | 1.State Key Lab of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China 2.College of Information and Electrical Engineering, Shenyang Agricultural University, Shenyang 110866, China 3.Department of Mechanical Engineering, University of Arkansas, Fayetteville, AR 72701, United States 4.Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, United States |
| 推荐引用方式 GB/T 7714 | Tung, Steve,Li, Yanbin,Harris, Nathan,et al. 3D-printed pneumatic microfluidic mixer for colorimetric detection of Listeria monocytogenes[C]. 见:. Detroit, MI, United states. July 29 - August 1, 2018. |
入库方式： OAI收割
来源：沈阳自动化研究所
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