Quantifying The Adhesion Forces of Lymphoma Cells by AFM Single-cell Force Spectroscopy
文献类型：期刊论文
| 作者 | Xiang RW(项荣武)4; Liu B(刘斌)1,3 ; Liu XF (刘小菲)4; Li M(李密)1,3; Dang D(党丹)2,4
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| 刊名 | 生物化学与生物物理进展
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| 出版日期 | 2019 |
| 卷号 | 46期号:1页码:89-98 |
| 关键词 | atomic force microscopy single-cell force spectroscopy cellular adhesion lymphoma cell rituximab |
| ISSN号 | 1000-3282 |
| 其他题名 | 基于AFM单细胞力谱技术的淋巴瘤细胞黏附力测量 |
| 产权排序 | 3 |
| 英文摘要 | Cell adhesion plays an important role in regulating diverse physiological functions of cells,and quantitatively characterizing the adhesive behaviors at single-cell level benefits understanding the biology of cells.The advent of atomic force microscopy(AFM)provides a powerful method for investigating the biophysical properties of biological systems at micro/nanoscale under aqueous conditions,and particularly AFM-based single-cell force spectroscopy(SCFS)is able to measure the adhesion forces of single cells.Nevertheless,current SCFS assays are commonly performed on adherent cells,and SCFS studies on mammalian suspended cells are still scarce.In this work,AFM-based SCFS was utilized to measure the adhesion forces of lymphoma cells.First, the adhesion forces between lymphoma cells and rituximab(an antibody which binds to the CD20antigen on lymphoma cells to activate immunotherapy)were investigated.Then the effects of antibody concentration and experimental parameters on the adhesion force measurements were investigated.Next,the intercellular adhesion forces between lymphoma cells were quantified.The research demonstrates the capabilities of AFM-based SCFS in detecting the adhesive behaviors of mammalian suspended cells and also provides novel insights into the adhesion of lymphoma cells,which will have potential impacts on single-cell biomechanical assays. |
| WOS关键词 | OPTICAL TWEEZERS ; MICROSCOPY ; MOLECULES |
| 资助项目 | National Natural Science Foundation of China[61433017] ; National Natural Science Foundation of China[61503372] ; National Natural Science Foundation of China[61873258] ; State Key Laboratory of Robotics[2016-O10] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| CSCD记录号 | CSCD:6413616 |
| WOS记录号 | WOS:000457065800010 |
| 源URL | [http://ir.sia.cn/handle/173321/24133]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 通讯作者 | Liu B(刘斌); Li M(李密); Dang D(党丹) |
| 作者单位 | 1.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110016, China 2.College of Mechanical Engineering, Northeastern University, Shenyang 110819, China 3.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China 4.School of Medical devices, Shenyang Pharmaceutical University, Shenyang 110016, China |
| 推荐引用方式 GB/T 7714 | Xiang RW,Liu B,Liu XF ,et al. Quantifying The Adhesion Forces of Lymphoma Cells by AFM Single-cell Force Spectroscopy[J]. 生物化学与生物物理进展,2019,46(1):89-98. |
| APA | Xiang RW,Liu B,Liu XF ,Li M,&Dang D.(2019).Quantifying The Adhesion Forces of Lymphoma Cells by AFM Single-cell Force Spectroscopy.生物化学与生物物理进展,46(1),89-98. |
| MLA | Xiang RW,et al."Quantifying The Adhesion Forces of Lymphoma Cells by AFM Single-cell Force Spectroscopy".生物化学与生物物理进展 46.1(2019):89-98. |
入库方式： OAI收割
来源：沈阳自动化研究所
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