EscC is a chaperone for the Edwardsiella tarda type III secretion system putative translocon components EseB and EseD
文献类型：期刊论文
| 作者 | Zheng, Jun; Li, Nan; Tan, Yuen Peng; Sivaraman, J.; Mok, Yu-Keung; Mo, Zhao Lan; Leung, Ka Yin |
| 刊名 | MICROBIOLOGY-SGM
 |
| 出版日期 | 2007-06-01 |
| 卷号 | 153期号:Part 6页码:1953-1962 |
| 关键词 | Enteropathogenic Escherichia-coli Salmonella Pathogenicity Island-2 Virulence Genes Proteins Bacteria Export Espb Fish Identification Pathogenesis |
| ISSN号 | 1350-0872 |
| DOI | 10.1099/mic.0.2006/004592-0 |
| 文献子类 | Article |
| 英文摘要 | Edwardsiella tarda is a Gram-negative enteric pathogen that causes disease in both humans and animals. Recently, a type III secretion system (T3SS) has been found to contribute to Ed. tarda pathogenesis. EseB, EseC and EseD were shown to be secreted by the T3SS and to be the major components of the extracellular proteins (ECPs). Based on sequence similarity, they have been proposed to function as the 'translocon' of the T3SS needle structure. In this study, it was shown that EseB, EseC and EseD formed a protein complex after secretion, which is consistent with their possible roles as translocon components. The secretion of EseB and EseD was dependent on EscC (previously named Orf2). EscC has the characteristics of a chaperone; it is a small protein (13 kDa), located next to the translocators in the T3SS gene cluster, and has a coiled-coil structure at the N-terminal region as predicted by COILS. An in-frame deletion of escC abolished the secretion of EseB and EseD, and complementation of Delta escC restored the export of EseB and EseD into the culture supernatant. Further studies showed that EscC is not a secreted protein and is located on the membrane and in the cytoplasm. Mutation of escC did not affect the transcription of eseB but reduced the amount of EseB as measured by using an EseB-LacZ fusion protein in Ed. tarda. Co-purification studies demonstrated that EscC formed complexes with EseB and EseD. The results suggest that EscC functions as a T3SS chaperone for the putative translocon components EseB and EseD in Ed. tarda.; Edwardsiella tarda is a Gram-negative enteric pathogen that causes disease in both humans and animals. Recently, a type III secretion system (T3SS) has been found to contribute to Ed. tarda pathogenesis. EseB, EseC and EseD were shown to be secreted by the T3SS and to be the major components of the extracellular proteins (ECPs). Based on sequence similarity, they have been proposed to function as the 'translocon' of the T3SS needle structure. In this study, it was shown that EseB, EseC and EseD formed a protein complex after secretion, which is consistent with their possible roles as translocon components. The secretion of EseB and EseD was dependent on EscC (previously named Orf2). EscC has the characteristics of a chaperone; it is a small protein (13 kDa), located next to the translocators in the T3SS gene cluster, and has a coiled-coil structure at the N-terminal region as predicted by COILS. An in-frame deletion of escC abolished the secretion of EseB and EseD, and complementation of Delta escC restored the export of EseB and EseD into the culture supernatant. Further studies showed that EscC is not a secreted protein and is located on the membrane and in the cytoplasm. Mutation of escC did not affect the transcription of eseB but reduced the amount of EseB as measured by using an EseB-LacZ fusion protein in Ed. tarda. Co-purification studies demonstrated that EscC formed complexes with EseB and EseD. The results suggest that EscC functions as a T3SS chaperone for the putative translocon components EseB and EseD in Ed. tarda. |
| 学科主题 | Microbiology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000247304900027 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5767]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Natl Univ Singapore, Fac Sci, Dept Biol Sci, Singapore 117543, Singapore 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Jun,Li, Nan,Tan, Yuen Peng,et al. EscC is a chaperone for the Edwardsiella tarda type III secretion system putative translocon components EseB and EseD[J]. MICROBIOLOGY-SGM,2007,153(Part 6):1953-1962. |
| APA | Zheng, Jun.,Li, Nan.,Tan, Yuen Peng.,Sivaraman, J..,Mok, Yu-Keung.,...&Leung, Ka Yin.(2007).EscC is a chaperone for the Edwardsiella tarda type III secretion system putative translocon components EseB and EseD.MICROBIOLOGY-SGM,153(Part 6),1953-1962. |
| MLA | Zheng, Jun,et al."EscC is a chaperone for the Edwardsiella tarda type III secretion system putative translocon components EseB and EseD".MICROBIOLOGY-SGM 153.Part 6(2007):1953-1962. |
入库方式： OAI收割
来源：海洋研究所
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