Chromosomal mapping of the major ribosomal RNA genes in the dwarf surfclam (Mulinia lateralis Say)
文献类型：期刊论文
| 作者 | Wang, Yongping1,2; Guo, Ximing1 |
| 刊名 | JOURNAL OF SHELLFISH RESEARCH
 |
| 出版日期 | 2008-04-01 |
| 卷号 | 27期号:2页码:307-311 |
| 关键词 | Fish Chromosomes Major Rrna Genes Genome Duplication Mulinia Lateralis |
| ISSN号 | 0730-8000 |
| 文献子类 | Article |
| 英文摘要 | Chromosomal location of the major ribosomal RNA genes (rRNA) were studied in the dwarf surfclam (Mulinia lateralis, Say) using fluorescence in situ hybridization (FISH). FISH probes for the rRNA genes were made by polymerase chain reaction (PCR), labeled with digoxigenin-11-dUTP and detected with fluorescein-labeled antidigoxigenin antibodies. Mulinia lateralis had a diploid number of 38 chromosomes and all chromosomes were telocentric. FISH with the rRNA probe produced positive and consistent signals on two pairs of chromosomes: Chromosome 15 with a relative length of 4.6% and Chromosome 19, the shortest chromosome. Both loci were telomeric. The rRNA location provides the first physical landmark of the M. lateralis genome.; Chromosomal location of the major ribosomal RNA genes (rRNA) were studied in the dwarf surfclam (Mulinia lateralis, Say) using fluorescence in situ hybridization (FISH). FISH probes for the rRNA genes were made by polymerase chain reaction (PCR), labeled with digoxigenin-11-dUTP and detected with fluorescein-labeled antidigoxigenin antibodies. Mulinia lateralis had a diploid number of 38 chromosomes and all chromosomes were telocentric. FISH with the rRNA probe produced positive and consistent signals on two pairs of chromosomes: Chromosome 15 with a relative length of 4.6% and Chromosome 19, the shortest chromosome. Both loci were telomeric. The rRNA location provides the first physical landmark of the M. lateralis genome. |
| 语种 | 英语 |
| WOS记录号 | WOS:000254880600010 |
| 公开日期 | 2010-12-30 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/6327]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA 2.Chinese Acad Sci, Inst Oceanol, Expt Marine Biiol Lab, Qingdao 266071, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yongping,Guo, Ximing. Chromosomal mapping of the major ribosomal RNA genes in the dwarf surfclam (Mulinia lateralis Say)[J]. JOURNAL OF SHELLFISH RESEARCH,2008,27(2):307-311. |
| APA | Wang, Yongping,&Guo, Ximing.(2008).Chromosomal mapping of the major ribosomal RNA genes in the dwarf surfclam (Mulinia lateralis Say).JOURNAL OF SHELLFISH RESEARCH,27(2),307-311. |
| MLA | Wang, Yongping,et al."Chromosomal mapping of the major ribosomal RNA genes in the dwarf surfclam (Mulinia lateralis Say)".JOURNAL OF SHELLFISH RESEARCH 27.2(2008):307-311. |
入库方式： OAI收割
来源：海洋研究所
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