Rapid and specific detection of all known nipah virus strains' sequences with reverse transcription-loop-mediated isothermal amplification
文献类型：期刊论文
| 作者 | Ma, Liping1,2,3; Chen, Zhen4; Guan, Wuxiang4; Chen, Quanjiao1; Liu, Di2,3 |
| 刊名 | Frontiers in microbiology
 |
| 出版日期 | 2019-03-11 |
| 卷号 | 10页码:10 |
| ISSN号 | 1664-302X |
| 关键词 | Nipah virus Reverse transcription-loop-mediated isothermal amplification Rt-lamp Rapid detection N gene |
| DOI | 10.3389/fmicb.2019.00418 |
| 通讯作者 | Chen, quanjiao(chenqj@wh.iov.cn) ; Liu, di(liud@wh.iov.cn) |
| 英文摘要 | Nipah virus (niv) is a zoonotic virus and can be transmitted through contaminated food or directly between people. niv is classified as a biosafety level 4 agent, not only because of its relatively high case fatality rate, but also because there is no vaccine or other medical countermeasures and it appears to be transmitted by fomites/particulates. the development of rapid detection assay for niv is of great importance because no effective field test is currently available. in this study, an isothermal (65 degrees c) reverse transcription-loop-mediated isothermal amplification (rt-lamp) method was developed, targeting the nucleocapsid protein (n) gene, for the rapid detection of niv, and was compared with conventional rt-pcr. three pseudoviruses of niv n gene representing all known strains were constructed to replace live niv. a set of rt-lamp primers, targeting a highly conserved region of the n gene in the viral genome was designed to identify all known niv strains. sensitivity tests indicated that the detection limit of the rt-lamp assay was approximately 100 pg of total niv pseudovirus rna, which is at least 10-fold higher than that of conventional rt-pcr. specificity tests showed that there was no cross-reactivity with nucleocapsid protein gene of hendra virus, newcastle disease virus, japanese encephalitis virus, or influenza a virus. the rt-lamp assay provides results within 45 min, and requires no sophisticated instruments, except an isothermal water bath or metal bath with 1 mu l calcein indicator. an analysis of the clinical samples showed that the assay had good stability. in conclusion, systematic experiments have shown that the rt-lamp assay developed here effectively detects three niv pseudoviruses representing all known strains of niv, with high specificity, sensitivity and stability. |
| WOS关键词 | HENDRA VIRUS ; ENCEPHALITIS ; BANGLADESH ; TRANSMISSION ; INFECTION ; DIAGNOSIS ; ASSAY ; LAMP |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| 出版者 | FRONTIERS MEDIA SA |
| WOS记录号 | WOS:000460774900001 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373173 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Quanjiao; Liu, Di |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, CAS Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, Computat Virol Grp, Wuhan Inst Virol, Wuhan, Hubei, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Ctr Emerging Infect Dis, Wuhan Inst Virol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Liping,Chen, Zhen,Guan, Wuxiang,et al. Rapid and specific detection of all known nipah virus strains' sequences with reverse transcription-loop-mediated isothermal amplification[J]. Frontiers in microbiology,2019,10:10. |
| APA | Ma, Liping,Chen, Zhen,Guan, Wuxiang,Chen, Quanjiao,&Liu, Di.(2019).Rapid and specific detection of all known nipah virus strains' sequences with reverse transcription-loop-mediated isothermal amplification.Frontiers in microbiology,10,10. |
| MLA | Ma, Liping,et al."Rapid and specific detection of all known nipah virus strains' sequences with reverse transcription-loop-mediated isothermal amplification".Frontiers in microbiology 10(2019):10. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。