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Single-particle tracking reveals the sequential entry process of the bunyavirus severe fever with thrombocytopenia syndrome virus

文献类型：期刊论文

作者Liu, Jia1; Xu, Mingyue2; Tang, Bo3,4; Hu, Liangbo2; Deng, Fei2; Wang, Hualin2; Pang, Dai-Wen3,4; Hu, Zhihong2; Wang, Manli2; Zhou, Yiwu1
刊名Small
出版日期2019-02-01
卷号15期号:6页码:16
关键词Clathrin Membrane fusion Quantum dots Severe fever with thrombocytopenia syndrome virus (sftsv) Single-particle tracking
ISSN号1613-6810
DOI10.1002/smll.201803788
通讯作者Wang, manli(wangml@wh.iov.cn) ; Zhou, yiwu(zhouyiwu@hust.edu.cn)
英文摘要The bunyavirales is one of the largest groups of rna viruses, which encompasses many strains that are highly pathogenic to animals and humans. severe fever with thrombocytopenia syndrome virus (sftsv) is an emerging tick-borne bunyavirus that causes severe disease in humans, with a high fatality rate of up to 30%. to date, the entry process of bunyavirus infection remains obscure. here, using quantum dot (qd)-based single-particle tracking and multicolor imaging, the dynamic molecular process of sftsv entry and penetration is systematically dissected. the results show that internalization of sftsv into host cells is initiated by recruiting clathrin onto the cell membrane for the formation of clathrin-coated pits and further pinching off from the plasma membrane to form discrete vesicles. these vesicular carriers further deliver virions to rab5+ early endosomes, and then to rab7+ late endosomes. the intracellular transport of virion-carrying endocytic vesicles is dependent first on actin filaments at the cell periphery, and then on microtubules toward the cell interior. the final fusion events occur at approximate to 15-60 min post-entry, and are triggered by the acidic environment at approximate to ph5.6 within the late endosomes. these results reveal the multistep sftsv entry process and the dynamic virus-host interactions involved.
WOS关键词CLATHRIN-MEDIATED ENDOCYTOSIS ; INFLUENZA-VIRUS ; HOST-CELLS ; DEPENDENT ENTRY ; DC-SIGN ; IDENTIFICATION ; TRAFFICKING ; MECHANISM ; INFECTION ; RECEPTOR
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS类目Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
语种英语
WOS记录号WOS:000458313900008
出版者WILEY-V C H VERLAG GMBH
URI标识http://www.irgrid.ac.cn/handle/1471x/2373193
专题武汉病毒研究所
通讯作者Wang, Manli; Zhou, Yiwu
作者单位1.Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Forens Med, Wuhan 430030, Hubei, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China
3.Wuhan Univ, Coll Chem & Mol Sci, State Key Lab Virol, Key Lab Analyt Chem Biol & Med,Minist Educ, Wuhan 430072, Hubei, Peoples R China
4.Wuhan Univ, Inst Adv Studies, Wuhan 430072, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Jia,Xu, Mingyue,Tang, Bo,et al. Single-particle tracking reveals the sequential entry process of the bunyavirus severe fever with thrombocytopenia syndrome virus[J]. Small,2019,15(6):16.
APA Liu, Jia.,Xu, Mingyue.,Tang, Bo.,Hu, Liangbo.,Deng, Fei.,...&Zhou, Yiwu.(2019).Single-particle tracking reveals the sequential entry process of the bunyavirus severe fever with thrombocytopenia syndrome virus.Small,15(6),16.
MLA Liu, Jia,et al."Single-particle tracking reveals the sequential entry process of the bunyavirus severe fever with thrombocytopenia syndrome virus".Small 15.6(2019):16.

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来源：武汉病毒研究所

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