An improved method for identifying sumoylation sites of viral proteins
文献类型：期刊论文
| 作者 | Liu, Yan1; Tan, Zhongyuan1; Shu, Bo1; Zhang, Yuan1; Zheng, Caishang1; Ke, Xianliang1; Chen, Xi2; Wang, Hanzhong1; Zheng, Zhenhua1 |
| 刊名 | Virologica sinica
 |
| 出版日期 | 2017-12-01 |
| 卷号 | 32期号:6页码:537-540 |
| ISSN号 | 1674-0769 |
| DOI | 10.1007/s12250-017-4018-3 |
| 通讯作者 | Zheng, zhenhua(zhengzh@wh.iov.cn) |
| 英文摘要 | In this study, we improved the most commonly used methods for ms detection of sumoylated sites and used an e. coli recombination sumoylation system with sumo-1 (t95r). this system provides fast enrichment of sumoylated viral protein in less than 2 days, and shows advantage over the method of collecting modified protein from cells in convenience and sensitivity. furthermore, this method provides an option for rapid and accurate identification of the potential viral protein sumoylation sites. |
| WOS关键词 | IN-VIVO IDENTIFICATION ; MASS-SPECTROMETRY ; SUMO MODIFICATION ; UBIQUITIN ; PATHWAYS ; STRATEGY ; VITRO |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000419186700011 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373347 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zheng, Zhenhua |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, CAS Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 2.Wuhan Inst Biotechnol, Wuhan 430075, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yan,Tan, Zhongyuan,Shu, Bo,et al. An improved method for identifying sumoylation sites of viral proteins[J]. Virologica sinica,2017,32(6):537-540. |
| APA | Liu, Yan.,Tan, Zhongyuan.,Shu, Bo.,Zhang, Yuan.,Zheng, Caishang.,...&Zheng, Zhenhua.(2017).An improved method for identifying sumoylation sites of viral proteins.Virologica sinica,32(6),537-540. |
| MLA | Liu, Yan,et al."An improved method for identifying sumoylation sites of viral proteins".Virologica sinica 32.6(2017):537-540. |
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来源：武汉病毒研究所
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