Tracking single baculovirus retrograde transportation in host cell via quantum dot-labeling of virus internal component
文献类型：期刊论文
| 作者 | Wen, Li1,2; Zheng, Zhen-Hua3; Liu, An-An1,2; Lv, Cheng1,2; Zhang, Li-Juan1,2; Ao, Jian1,2; Zhang, Zhi-Ling1,2; Wang, Han-Zhong3; Lin, Yi1,2; Pang, Dai-Wen1,2,3 |
| 刊名 | Journal of nanobiotechnology
 |
| 出版日期 | 2017-05-06 |
| 卷号 | 15页码:10 |
| 关键词 | Quantum dots Single virus tracking Baculovirus Retrograde transportation Host cells |
| ISSN号 | 1477-3155 |
| DOI | 10.1186/s12951-017-0270-9 |
| 通讯作者 | Lin, yi(ylin@whu.edu.cn) |
| 英文摘要 | Background: quantum dot (qd)-based single virus tracking has become a powerful tool for dissecting virus infection mechanism. however, only virus behaviors at the early stage of retrograde trafficking have been dynamically tracked so far. monitoring of comprehensive virus retrograde transportation remains a challenge. results: based on the superior fluorescence properties of qds and their labeling of virus internal component, the dynamic interactions between baculoviruses and all key transportation-related cellular structures, including vesicles, acidic endosomes, actins, nuclear pores and nuclei, were visualized at the single-virus level. detailed scenarios and dynamic information were provided for these critical interaction processes. conclusions: a comprehensive model of baculovirus retrograde trafficking involving virus endocytosis, fusion with acidic endosome, translocation to nuclear periphery, internalization into nucleus, and arriving at the destination in nucleus was proposed. thus the whole retrograde transportation of baculovirus in live host cells was elucidated at the single-virus level for the first time. |
| WOS关键词 | CLATHRIN-MEDIATED ENDOCYTOSIS ; INFLUENZA-VIRUS ; PARTICLE TRACKING ; NANOPARTICLES ; INFECTION ; ENVELOPE ; ENTRY |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Science & Technology - Other Topics |
| WOS类目 | Biotechnology & Applied Microbiology ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| WOS记录号 | WOS:000402010100001 |
| 出版者 | BIOMED CENTRAL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373439 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Lin, Yi |
| 作者单位 | 1.Wuhan Univ, Key Lab Analyt Chem Biol & Med, Coll Chem & Mol Sci, Minist Educ,State Key Lab Virol,Inst Adv Studies, Wuhan 430072, Peoples R China 2.Wuhan Univ, Wuhan Inst Biotechnol, Wuhan 430072, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wen, Li,Zheng, Zhen-Hua,Liu, An-An,et al. Tracking single baculovirus retrograde transportation in host cell via quantum dot-labeling of virus internal component[J]. Journal of nanobiotechnology,2017,15:10. |
| APA | Wen, Li.,Zheng, Zhen-Hua.,Liu, An-An.,Lv, Cheng.,Zhang, Li-Juan.,...&Pang, Dai-Wen.(2017).Tracking single baculovirus retrograde transportation in host cell via quantum dot-labeling of virus internal component.Journal of nanobiotechnology,15,10. |
| MLA | Wen, Li,et al."Tracking single baculovirus retrograde transportation in host cell via quantum dot-labeling of virus internal component".Journal of nanobiotechnology 15(2017):10. |
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来源：武汉病毒研究所
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