Divergent transactivation of maize storage protein zein genes by the transcription factors opaque2 and ohps
文献类型：期刊论文
| 作者 | Yang, Jun1; Ji, Chen1,2; Wu, Yongrui1 |
| 刊名 | Genetics
 |
| 出版日期 | 2016-10-01 |
| 卷号 | 204期号:2页码:581-+ |
| 关键词 | Maize Endosperm Storage proteins Transcription factors Subfunctionalization |
| ISSN号 | 0016-6731 |
| DOI | 10.1534/genetics.116.192385 |
| 通讯作者 | Wu, yongrui(yrwu@sibs.ac.cn) |
| 英文摘要 | Maize transcription factors (tfs) opaque2 (o2) and the o2 heterodimerizing proteins (ohp1 and ohp2) originated from an ancient segmental duplication. the 22-kda (z1c) and 19-kda (z1a, z1b, and z1d) alpha-zeins are the most abundant storage proteins in maize endosperm. o2 is known to regulate alpha-zein gene expression, but its target motifs in the 19-kda alpha-zein gene promoters have not been identified. the mechanisms underlying the regulation of alpha-zein genes by these tfs are also not well understood. in this study, we found that the o2 binding motifs in the alpha-zein gene promoters are quite flexible, with acgt being present in the z1c and z1a promoters and a variant, acat, being present in the z1b and z1d promoters. ohps recognized and transactivated all of the alpha-zein promoters, although to much lower levels than did o2. in the presence of o2, the suppression of ohps did not cause a significant reduction in the transcription of alpha-zein genes, but in the absence of o2, ohps were critical for the expression of residual levels of alpha-zeins. these findings demonstrated that o2 is the primary tf and that ohps function as minor tfs in this process. this relationship is the converse of that involved in 27-kda gamma-zein gene regulation, indicating that the specificities of o2 and the ohps for regulating zein genes diverged after gene duplication. the prolamine-box binding factor by itself has limited transactivation activity, but it promotes the binding of o2 to o2 motifs, resulting in the synergistic transactivation of alpha-zein genes. |
| WOS关键词 | DNA-BINDING SPECIFICITIES ; PROMOTER ACTIVITY ; GCN4-LIKE MOTIF ; ENDOSPERM BOX ; BZIP PROTEIN ; PROLAMIN BOX ; EXPRESSION ; RICE ; ACTIVATOR ; PLANT |
| WOS研究方向 | Genetics & Heredity |
| WOS类目 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000385871400017 |
| 出版者 | GENETICS SOCIETY AMERICA |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374895 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wu, Yongrui |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Natl Key Lab Plant Mol Genet, Ctr Excellence Mol Plant Sci,Inst Plant Physiol &, Shanghai 200032, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Jun,Ji, Chen,Wu, Yongrui. Divergent transactivation of maize storage protein zein genes by the transcription factors opaque2 and ohps[J]. Genetics,2016,204(2):581-+. |
| APA | Yang, Jun,Ji, Chen,&Wu, Yongrui.(2016).Divergent transactivation of maize storage protein zein genes by the transcription factors opaque2 and ohps.Genetics,204(2),581-+. |
| MLA | Yang, Jun,et al."Divergent transactivation of maize storage protein zein genes by the transcription factors opaque2 and ohps".Genetics 204.2(2016):581-+. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。