Sigma(e)-dependent activation of rbpa controls transcription of the fura-katg operon in response to oxidative stress in mycobacteria
文献类型：期刊论文
| 作者 | Hu, Yangbo1; Wang, Zhongwei1,2; Feng, Lipeng1,2; Chen, Zhenkang1,2; Mao, Chunyou1,2; Zhu, Yan1,2; Chen, Shiyun1 |
| 刊名 | Molecular microbiology
 |
| 出版日期 | 2016-10-01 |
| 卷号 | 102期号:1页码:107-120 |
| ISSN号 | 0950-382X |
| DOI | 10.1111/mmi.13449 |
| 通讯作者 | Chen, shiyun(sychen@wh.iov.cn) |
| 英文摘要 | Mycobacterium tuberculosis adopts various strategies to cope with oxidative stress during infection. transcriptional regulators, including sigma factors, make important contributions to this stress response, but how these proteins cooperate with each other is largely unknown. in this study, the role of rbpa and its cooperation with sigma factors in response to oxidative stress are investigated. knock down expression of rbpa in mycobacterium smegmatis attenuated bacterial survival in the presence of h2o2. additionally, transcription of the rbpa gene was induced by h2o2 in a sigma(e)-dependent manner. after induction, rbpa interacts with the principal sigma factor, sigma(a), to control the transcription of fura-katg operon, which encodes an h2o2 scavenging enzyme. moreover, this regulation is responsible for the role of sigma(e) in oxidative response because bacterial survival was attenuated and transcription of the fura-katg operon was down-regulated with h2o2 treatment in sige deletion mutant (sige), and over-expression of rbpa in sige strain restored all of these phenotypes. taken together, our study first illustrated a mechanism for sigma(e) in response to oxidative stress through regulation of rbpa transcription. this study was also the first to demonstrate that rbpa is required for the full response to oxidative stress by cooperating with the principal sigma(a). |
| WOS关键词 | SIGMA-FACTOR ; RNA-POLYMERASE ; BINDING PROTEIN ; STREPTOMYCES-COELICOLOR ; FLP RECOMBINASE ; GENE-EXPRESSION ; IN-VIVO ; TUBERCULOSIS ; SMEGMATIS ; SURVIVAL |
| WOS研究方向 | Biochemistry & Molecular Biology ; Microbiology |
| WOS类目 | Biochemistry & Molecular Biology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000384807900008 |
| 出版者 | WILEY-BLACKWELL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375051 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen, Shiyun |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Key Lab Special Pathogens & Biosafety, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10086, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Yangbo,Wang, Zhongwei,Feng, Lipeng,et al. Sigma(e)-dependent activation of rbpa controls transcription of the fura-katg operon in response to oxidative stress in mycobacteria[J]. Molecular microbiology,2016,102(1):107-120. |
| APA | Hu, Yangbo.,Wang, Zhongwei.,Feng, Lipeng.,Chen, Zhenkang.,Mao, Chunyou.,...&Chen, Shiyun.(2016).Sigma(e)-dependent activation of rbpa controls transcription of the fura-katg operon in response to oxidative stress in mycobacteria.Molecular microbiology,102(1),107-120. |
| MLA | Hu, Yangbo,et al."Sigma(e)-dependent activation of rbpa controls transcription of the fura-katg operon in response to oxidative stress in mycobacteria".Molecular microbiology 102.1(2016):107-120. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。