Simultaneous determination of six phenolic constituents of danshen in human serum using liquid chromatography/tandem mass spectrometry
文献类型：期刊论文
| 作者 | Li, XC; Yu, C; Cai, YB; Liu, GY; Jia, JY; Wang, YP |
| 刊名 | Journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2005-06-05 |
| 卷号 | 820期号:1页码:41-47 |
| 关键词 | Magnesium lithospermate b Rosmarinic acid Lithospermic acid Caffeic acid Protocatechuic aldehyde Danshensu Danshen Pharmacokinetics |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2005.03.005 |
| 通讯作者 | Li, xc(xchli@sibs.ac.cn) |
| 英文摘要 | The six phenolic constituents are water-soluble components extracted from the chinese medical herb danshen, the dried roots of salvia miltiorthiza bunge (labiatae). an liquid chromatography/tandem mass spectrometry (lc/ms/ms)-bascd method has been developed for the simultaneous quantification of six phenolic constituents of danshen (magnesium lithospermate b (mlb), rosmarinic acid (ra) and lithospermic acid (la), caffeic acid (caa), protocatechuic aldehyde (3,4-dihydroxybenzaldehyde, pal), 3,4-dihydroxyphenyllactic acid (danshensu)) in human serum with chloramphenicol as internal standard. the serum samples were treated by special liquid-liquid extraction, and the analytes were determined using electrospray negative ionization mass spectrometry in the multiple reaction monitoring (mrm) mode, with sufficient sensitivity to allow analysis of human serum samples generated following administration of a clinically relevant dose. good linearity over the range 8-2048 ng/ml for six phenolic constituents was observed. the intra- and inter-day precisions (cv) of analysis were < 13%, and the accuracy ranged from 88 to 116%. this quantitation method was successfully applied to a pharmacokinetic study of i.v. drip infusion of danshen injection fluid in human. © 2005 elsevier b.v. all rights reserved. |
| WOS关键词 | MAGNESIUM LITHOSPERMATE-B ; SALVIA-MILTIORRHIZA ; ROSMARINIC ACID |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000229167000006 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375209 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Li, XC |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Dept Pharmacol, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China 3.Shanghui Xuhui Cent Hosp, Cent Lab, Shanghai 200002, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, XC,Yu, C,Cai, YB,et al. Simultaneous determination of six phenolic constituents of danshen in human serum using liquid chromatography/tandem mass spectrometry[J]. Journal of chromatography b-analytical technologies in the biomedical and life sciences,2005,820(1):41-47. |
| APA | Li, XC,Yu, C,Cai, YB,Liu, GY,Jia, JY,&Wang, YP.(2005).Simultaneous determination of six phenolic constituents of danshen in human serum using liquid chromatography/tandem mass spectrometry.Journal of chromatography b-analytical technologies in the biomedical and life sciences,820(1),41-47. |
| MLA | Li, XC,et al."Simultaneous determination of six phenolic constituents of danshen in human serum using liquid chromatography/tandem mass spectrometry".Journal of chromatography b-analytical technologies in the biomedical and life sciences 820.1(2005):41-47. |
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来源：武汉病毒研究所
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