Fluorometric broad-range screening of compounds with affinity for nucleic acids
文献类型：期刊论文
| 作者 | Liu, Y; Danielsson, B |
| 刊名 | Analytical chemistry
 |
| 出版日期 | 2005-04-15 |
| 卷号 | 77期号:8页码:2450-2454 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac048449o |
| 通讯作者 | Danielsson, b() |
| 英文摘要 | The potential of a nucleic acid-based optical bioprobe for environmental measurements and drug monitoring is described. the sensor employs the long-wavelength intercalating fluorophore to-pro-3 (tp3). compounds that interact with the tp3-dna complex are indirectly detected by a decrease in the fluorescence intensity. we found that the configuration and length of the dna dramatically affected the intensity of the fluorescence emitted from the tp3-dna complex. we compared nucleic acids from different sources and optimized the system for pbr322 plasmid dna (4363 bp) digested by hindiii restriction endonuclease. this endonuclease has a single recognition site in plasmid pbr322. in the proposed method, we attempt to combine broad-range detection with rapid and simple operation. a fiber-optic capillary fluorescence system was used to analyze toxic aromatic amines, antibiotics, and several kinds of antitumor drugs, using small amounts of sample, down to 10 mu l, with a sensitivity comparable to that of current electrochemical methods. the detection limit can be as low as a few ppb or submicromolar. this approach is useful for routine screening in environmental monitoring or for controlling cytotoxic drug administration. the ease of operation and the rapid response allow high-throughput screening. |
| WOS关键词 | SYBR-GREEN-I ; DNA ; CHLORAMPHENICOL ; FLUORESCENCE ; ADRIAMYCIN ; BIOSENSOR ; RESIDUES ; SHRIMP ; CELLS |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000228605100023 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375210 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Danielsson, B |
| 作者单位 | 1.Lund Univ, SE-22100 Lund, Sweden 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Y,Danielsson, B. Fluorometric broad-range screening of compounds with affinity for nucleic acids[J]. Analytical chemistry,2005,77(8):2450-2454. |
| APA | Liu, Y,&Danielsson, B.(2005).Fluorometric broad-range screening of compounds with affinity for nucleic acids.Analytical chemistry,77(8),2450-2454. |
| MLA | Liu, Y,et al."Fluorometric broad-range screening of compounds with affinity for nucleic acids".Analytical chemistry 77.8(2005):2450-2454. |
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来源：武汉病毒研究所
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