Characterization and partial genome sequence analysis of clostera anachoreta granulovirus
文献类型：期刊论文
| 作者 | Zhang, XX; Liang, ZP; Peng, HY; Zhang, ZX; Tang, XC; Liu, TQ |
| 刊名 | Virus research
 |
| 出版日期 | 2005-10-01 |
| 卷号 | 113期号:1页码:36-43 |
| 关键词 | Clostera anachoreta granulovirus Sequence analysis Phylogenetic Morphological Restriction |
| ISSN号 | 0168-1702 |
| DOI | 10.1016/j.virusres.2005.04.013 |
| 通讯作者 | Peng, hy() |
| 英文摘要 | The morphological and biological properties as well as partial genomic sequencing of a granulovirus isolated from clostera anachoreta (lepidoptera: notodontidae), c. anachoreta granulovirus (clangv), were carried out. the ovoidal occlusion bodies were 337 mm x 170 nm in size, and each granule contained one single rod-shape virion, with a mean size of 250 nm x 46 nm. granulin had a molecular weight of approximately 30 kda. clangv genome size was estimated as 104.34 kb based on the restriction fragments. the restriction pattern of the clangv genome was different from other gvs. a restriction fragment genomic library of clangv genome was constructed. the library consisted of nine sali fragments, seven hindiii fragments and seven ecori fragments. one 4.8 kb fragment of the genome, digested by sali, was sequenced and analyzed. this region was composed of eight unknown orfs. two baculoviruses homologous gene (vp1054 and lef10) and partial sequence of lef-8. the unknown orfs included three unique to clangv, the other five orfs were related to baculoviruses. the orfs, located within this restriction fragment, were compared to homologues in other gvs. the results indicated that clangv, cpgv, clgv, aogv and pogv had similar arrangement and orientation of the homologous orfs. phylogenetic analysis of vp1054 proteins from 20 baculoviruses indicated that clangv was more closely related to cpgv, clgv, aogv and pogv than to other baculoviruses. (c) 2005 elsevier b.v. all rights reserved. |
| WOS关键词 | POMONELLA GRANULOSIS-VIRUS ; PHYSICAL MAP ; GENE REGION ; IDENTIFICATION |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000232022200004 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375211 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Peng, HY |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Lab Biol Control, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Henan Agr Univ, Coll Biol Tech & Food Sci, Zhengzhou 450002, Henan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, XX,Liang, ZP,Peng, HY,et al. Characterization and partial genome sequence analysis of clostera anachoreta granulovirus[J]. Virus research,2005,113(1):36-43. |
| APA | Zhang, XX,Liang, ZP,Peng, HY,Zhang, ZX,Tang, XC,&Liu, TQ.(2005).Characterization and partial genome sequence analysis of clostera anachoreta granulovirus.Virus research,113(1),36-43. |
| MLA | Zhang, XX,et al."Characterization and partial genome sequence analysis of clostera anachoreta granulovirus".Virus research 113.1(2005):36-43. |
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来源：武汉病毒研究所
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