Plasmid-borne catabolism of methyl parathion and p-nitrophenol in pseudomonas sp strain wbc-3
文献类型：期刊论文
| 作者 | Liu, H; Zhang, JJ; Wang, SJ; Zhang, XE; Zhou, NY |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2005-09-09 |
| 卷号 | 334期号:4页码:1107-1114 |
| 关键词 | Catabolic plasmid Degradation Hydrolase Methyl parathion Mpd Mph Opd Organophosphate P-nitrophenol Pseudomonas Transposon |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2005.07.006 |
| 通讯作者 | Zhou, ny(n.zhou@pentium.whiov.ac.cn) |
| 英文摘要 | Pseudomonas sp. strain wbc-3 utilises methyl parathion (mp) or p-nitrophenol (pnp) as the sole source of carbon, nitrogen, and energy. a plasmid designated pzwlo of approximate to 70 kb in this strain was found to be responsible for mp and pnp degradation. this was based on the fact that the plasmid-cured strains showed pnp(-)mp(-) phenotype and the pnp(+)mp(+) phenotype could be conjugally transferred. we have also cloned a 3.4-kb hindiii fragment which exhibited methyl parathion hydrolase activity, which revealed a methyl parathion hydrolase (mph) gene whose dna sequence is 99.5% identical to the recently identified mpd gene from plesiomonas sp. m6 [c. zhongli, l. shunpeng, f. guoping, isolation of methyl parathion-degrading strain m6 and cloning of the methyl parathion hydrolase gene, appl. environ. microbiol. 67 (2001) 4922-4925]. the mph gene was functionally expressed in escherichia coli and the relative activities of the enzyme against different substrates were determined. the sequence alignment and phylogenetic analysis suggested that mph and mpd evolved independently from other well-studied organophosphate hydrolases and may be originated from class b beta-lactamase family. subsequently obtained a 6.5-kb kpni and bamhi fragment containing the above hindiii fragment revealed that the mph gene was physically located in a typical transposon. (c) 2005 elsevier inc. all rights reserved. |
| WOS关键词 | STANDARD NUMBERING SCHEME ; METALLO-BETA-LACTAMASE ; FLAVOBACTERIUM SP ; OPD GENE ; ORGANOPHOSPHATE INSECTICIDES ; DEGRADATION GENE ; MORAXELLA SP ; TOL PLASMID ; PROTEIN ; PHOSPHOTRIESTERASE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000231190900018 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375212 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhou, NY |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, H,Zhang, JJ,Wang, SJ,et al. Plasmid-borne catabolism of methyl parathion and p-nitrophenol in pseudomonas sp strain wbc-3[J]. Biochemical and biophysical research communications,2005,334(4):1107-1114. |
| APA | Liu, H,Zhang, JJ,Wang, SJ,Zhang, XE,&Zhou, NY.(2005).Plasmid-borne catabolism of methyl parathion and p-nitrophenol in pseudomonas sp strain wbc-3.Biochemical and biophysical research communications,334(4),1107-1114. |
| MLA | Liu, H,et al."Plasmid-borne catabolism of methyl parathion and p-nitrophenol in pseudomonas sp strain wbc-3".Biochemical and biophysical research communications 334.4(2005):1107-1114. |
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来源：武汉病毒研究所
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