Arg(169) is essential for catalytic activity of 3-hydroxybenzoate 6-hydroxylase from klebsiella pneumoniae m5a1
文献类型：期刊论文
| 作者 | Liu, DQ; Liu, H; Gao, XL; Leak, DJ; Zhou, NY |
| 刊名 | Microbiological research
 |
| 出版日期 | 2005 |
| 卷号 | 160期号:1页码:53-59 |
| 关键词 | 3-hydroxybenzoate 6-hydroxylase Site-directed mutagenesis Klebsiella pneumoniae m5a1 |
| ISSN号 | 0944-5013 |
| DOI | 10.1016/j.micres.2004.09.003 |
| 通讯作者 | Zhou, ny() |
| 英文摘要 | 3-hydroxybenzoate 6-hydroxylase from klebsiella pneumoniae m5a1 is an enzyme that utilizes 3-hydroxybenzoate (3-hba) as substrate yielding gentisate. site-directed mutagenesis was carried out to define which residues may be involved in catalytic reaction. substitution of arginine to glutamate at position 169 of the enzyme resulted in the complete loss of catalytic activity. this indicated arg(169) may play an important role in 3-hba 6-hydroxylase catalysis. (c) 2004 elsevier gmbh. all rights reserved. |
| WOS关键词 | SP STRAIN U2 ; GENTISATE PATHWAY ; SALICYLATE ; PURIFICATION ; NAPHTHALENE ; HYDROXYBENZOATE ; HYDROXYLASE ; METABOLISM ; ALIGNMENT ; DATABASE |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000227727800006 |
| 出版者 | URBAN & FISCHER VERLAG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375250 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhou, NY |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.Univ London Imperial Coll Sci Technol & Med, Dept Biol Sci, London SW7 2AZ, England |
| 推荐引用方式 GB/T 7714 | Liu, DQ,Liu, H,Gao, XL,et al. Arg(169) is essential for catalytic activity of 3-hydroxybenzoate 6-hydroxylase from klebsiella pneumoniae m5a1[J]. Microbiological research,2005,160(1):53-59. |
| APA | Liu, DQ,Liu, H,Gao, XL,Leak, DJ,&Zhou, NY.(2005).Arg(169) is essential for catalytic activity of 3-hydroxybenzoate 6-hydroxylase from klebsiella pneumoniae m5a1.Microbiological research,160(1),53-59. |
| MLA | Liu, DQ,et al."Arg(169) is essential for catalytic activity of 3-hydroxybenzoate 6-hydroxylase from klebsiella pneumoniae m5a1".Microbiological research 160.1(2005):53-59. |
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来源：武汉病毒研究所
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