Cytolytic toxin cyt1aa of bacillus thuringiensis synergizes the mosquitocidal toxin mtx1 of bacillus sphaericus
文献类型：期刊论文
| 作者 | Zhang, Beihua; Liu, Ming; Yang, Yankun; Yuan, Zhiming |
| 刊名 | Bioscience biotechnology and biochemistry
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 70期号:9页码:2199-2204 |
| 关键词 | 100-kilodalton mosquitocidal toxin Synergism Bacillus sphaericus Cyt1aa Resistance |
| ISSN号 | 0916-8451 |
| DOI | 10.1271/bbb.60140 |
| 通讯作者 | Yuan, zhiming(yzm@pentium.whiov.ac.cn) |
| 英文摘要 | Using the shuttle vector pbu4, the mosquitocidal toxin gene mtx1 from bacillus sphaericus strain ssii-1 was introduced into an acrystalliferous strain of b. thuringiensis both individually and in combination with the accessory protein gene p20 and the cytolytic protein gene cyt1aa from b. thuringiensis subsp. israelensis. bioassay results indicated that the recombinants b-pmt4(mtx1) and b-pmt9(mtx1), both individually containing mtx1, had moderate toxicities to binary toxin susceptible and binary toxin resistant culex quinque-fasciatus larvae during the vegetative growth stage, but that their toxicities declined rapidly during the sporulation phase. the lc50 values were 2.5 and 4.8 mg/ml respectively, against 3-4 instar susceptible and resistant larvae for the final sporulated cultures of recombinants b-pmt9(mtx1), and little toxicity was detected for b-pmt4(mtx1). meanwhile, the recombinant b-pmpx2(mtx1+cyt1aa) expressing mtx1, p20 alone, and cyt1aa in combination had stable toxicities during both the vegetative phase and the sporulation phase, with a lc50 ranging from 0.45-0.58 mg/ml., furthermore, expression of cyt1aa appeared to enhance the activity of mtx1 to target mosquito larvae, suggesting a synergism between cyt1aa and mtx1 toxins. |
| WOS关键词 | CULEX-QUINQUEFASCIATUS ; SUBSP ISRAELENSIS ; RESISTANCE ; EXPRESSION ; PROTEIN ; GENE ; CLONING ; SSII-1 ; STRAIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry ; Food Science & Technology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry, Applied ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000240972400024 |
| 出版者 | TAYLOR & FRANCIS LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375311 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yuan, Zhiming |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Beihua,Liu, Ming,Yang, Yankun,et al. Cytolytic toxin cyt1aa of bacillus thuringiensis synergizes the mosquitocidal toxin mtx1 of bacillus sphaericus[J]. Bioscience biotechnology and biochemistry,2006,70(9):2199-2204. |
| APA | Zhang, Beihua,Liu, Ming,Yang, Yankun,&Yuan, Zhiming.(2006).Cytolytic toxin cyt1aa of bacillus thuringiensis synergizes the mosquitocidal toxin mtx1 of bacillus sphaericus.Bioscience biotechnology and biochemistry,70(9),2199-2204. |
| MLA | Zhang, Beihua,et al."Cytolytic toxin cyt1aa of bacillus thuringiensis synergizes the mosquitocidal toxin mtx1 of bacillus sphaericus".Bioscience biotechnology and biochemistry 70.9(2006):2199-2204. |
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来源：武汉病毒研究所
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