Detection and phylogenic analysis of one anthrax virulence plasmid pxo1 conservative open reading frame ubiquitous presented within bacillus cereus group strains
文献类型：期刊论文
| 作者 | Hu, Xiaomin; Hansen, Bjarne Munk; Hendriksen, Niels Bohse; Yuan, Zhiming |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2006-11-03 |
| 卷号 | 349期号:4页码:1214-1219 |
| 关键词 | Pxo1 Orf101 Bacillus cereus Bacillus thuringiensis Snp |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2006.08.125 |
| 通讯作者 | Yuan, zhiming(yzm@pentium.whiov.ac.cn) |
| 英文摘要 | The presence of one of the anthrax virulence plasmid pxo1 conserved fragments was analyzed in 24 bacillus cereus and b. thuringiensis strains, including 6 b. thuringiensis subspecies, by polymerase chain reactions. twelve out of 24 strains showed pcr-positive for an orf101 homologous sequence. two pxo1-orf101-like fragments from a b. cereus b-4ac and a commercial b. thuringiensis kurstaki hd1 were cloned, sequenced and expressed in escherichia coli. toxicity assays revealed that the product encoded by the pxo1-orf101-like fragment had no impact on either vero cells or chinese hamster ovary cells, suggesting that this fragment probably not contribute to enterotoxic activity. sequence alignment of the pxo1-orf1o1 from three bacillus anthracis and orf101-like fragments from other 12 b. cereus group isolates indicated high identity (more than 90%) and the presence of subgroup- and strain-specific snps among these fragments. (c) 2006 elsevier inc. all rights reserved. |
| WOS关键词 | SEQUENCE ; BCET ; KURSTAKI ; GENE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000241064800006 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375316 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yuan, Zhiming |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Natl Environm Res Inst, DK-4000 Roskilde, Denmark |
| 推荐引用方式 GB/T 7714 | Hu, Xiaomin,Hansen, Bjarne Munk,Hendriksen, Niels Bohse,et al. Detection and phylogenic analysis of one anthrax virulence plasmid pxo1 conservative open reading frame ubiquitous presented within bacillus cereus group strains[J]. Biochemical and biophysical research communications,2006,349(4):1214-1219. |
| APA | Hu, Xiaomin,Hansen, Bjarne Munk,Hendriksen, Niels Bohse,&Yuan, Zhiming.(2006).Detection and phylogenic analysis of one anthrax virulence plasmid pxo1 conservative open reading frame ubiquitous presented within bacillus cereus group strains.Biochemical and biophysical research communications,349(4),1214-1219. |
| MLA | Hu, Xiaomin,et al."Detection and phylogenic analysis of one anthrax virulence plasmid pxo1 conservative open reading frame ubiquitous presented within bacillus cereus group strains".Biochemical and biophysical research communications 349.4(2006):1214-1219. |
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来源：武汉病毒研究所
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