Molecular identification and phylogenetic analysis of baculoviruses from lepidoptera
文献类型：期刊论文
| 作者 | Jehle, JA; Lange, M; Wang, HL; Hu, ZH; Wang, YJ; Hauschild, W |
| 刊名 | Virology
 |
| 出版日期 | 2006-03-01 |
| 卷号 | 346期号:1页码:180-193 |
| 关键词 | Baculoviridae Nucleopolyhedrovirus Granulovirus Virus identification Molecular phylogeny Polymerase chain reaction |
| ISSN号 | 0042-6822 |
| DOI | 10.1016/j.virol.2005.10.032 |
| 通讯作者 | Jehle, ja(johannes.jehlc@dlr.rlp.de) |
| 英文摘要 | Pcr amplification of the highly conserved baculovirus genes late expression factor 8 (lef-8), late expression factor 9 (lef-9) and polyhedrin/granulin (polh/gran) combined with molecular phylogenetic analyses provide a powerful tool to identify lepidopteran-specific baculoviruses and to study their diversity. in the present investigation, we have improved the degenerate oligonucleotides and corroborated the approach that was recently described by lange et al. (lange, m., wang, h., zhihong, h., jehle, j.a., 2004. towards a molecular identification and classification system of lepidopteran-specific baculoviruses. virology 325, 36-47.). baculovirus dna was isolated from 71 uncharacterized historic baculovirus samples, and partial gene sequences were amplified by using gene-specific degenerate pcr primers. the obtained pcr products were directly sequenced, and the deduced amino acid sequences were compiled and aligned with published sequences of these target genes. a phylogenctic tree of 117 baculoviruses was inferred using maximum parsimony and distance methods. based on the comprehensive phylogenetic analysis of the partial lef-8, lef-9 and polh/gran genes, we propose a phylogenetic species criterion for lepidopteran-specific baculoviruses that uses the genetic distances of these genes for species demarcation. (c) 2005 elsevier inc. all rights reserved. |
| WOS关键词 | SINGLE NUCLEOCAPSID NUCLEOPOLYHEDROVIRUS ; GENOME SEQUENCE ; MAMESTRA-CONFIGURATA ; RACHIPLUSIA-OU ; GENE ; ORGANIZATION ; ALIGNMENT ; VIRUS |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000235930400017 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375319 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Jehle, JA |
| 作者单位 | 1.Agr Serv Ctr Palatinate, Lab Biotechnol Crop Protect, Dept Phytopathol, D-67435 Neustadt, Germany 2.Chinese Acad Sci, State Key Lab Virol, Wuhan 430071, Peoples R China 3.Chinese Acad Sci, Joint Lab Invertebrate Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jehle, JA,Lange, M,Wang, HL,et al. Molecular identification and phylogenetic analysis of baculoviruses from lepidoptera[J]. Virology,2006,346(1):180-193. |
| APA | Jehle, JA,Lange, M,Wang, HL,Hu, ZH,Wang, YJ,&Hauschild, W.(2006).Molecular identification and phylogenetic analysis of baculoviruses from lepidoptera.Virology,346(1),180-193. |
| MLA | Jehle, JA,et al."Molecular identification and phylogenetic analysis of baculoviruses from lepidoptera".Virology 346.1(2006):180-193. |
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来源：武汉病毒研究所
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