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Molecular identification and phylogenetic analysis of baculoviruses from lepidoptera

文献类型:期刊论文

作者Jehle, JA; Lange, M; Wang, HL; Hu, ZH; Wang, YJ; Hauschild, W
刊名Virology
出版日期2006-03-01
卷号346期号:1页码:180-193
关键词Baculoviridae Nucleopolyhedrovirus Granulovirus Virus identification Molecular phylogeny Polymerase chain reaction
ISSN号0042-6822
DOI10.1016/j.virol.2005.10.032
通讯作者Jehle, ja(johannes.jehlc@dlr.rlp.de)
英文摘要Pcr amplification of the highly conserved baculovirus genes late expression factor 8 (lef-8), late expression factor 9 (lef-9) and polyhedrin/granulin (polh/gran) combined with molecular phylogenetic analyses provide a powerful tool to identify lepidopteran-specific baculoviruses and to study their diversity. in the present investigation, we have improved the degenerate oligonucleotides and corroborated the approach that was recently described by lange et al. (lange, m., wang, h., zhihong, h., jehle, j.a., 2004. towards a molecular identification and classification system of lepidopteran-specific baculoviruses. virology 325, 36-47.). baculovirus dna was isolated from 71 uncharacterized historic baculovirus samples, and partial gene sequences were amplified by using gene-specific degenerate pcr primers. the obtained pcr products were directly sequenced, and the deduced amino acid sequences were compiled and aligned with published sequences of these target genes. a phylogenctic tree of 117 baculoviruses was inferred using maximum parsimony and distance methods. based on the comprehensive phylogenetic analysis of the partial lef-8, lef-9 and polh/gran genes, we propose a phylogenetic species criterion for lepidopteran-specific baculoviruses that uses the genetic distances of these genes for species demarcation. (c) 2005 elsevier inc. all rights reserved.
WOS关键词SINGLE NUCLEOCAPSID NUCLEOPOLYHEDROVIRUS ; GENOME SEQUENCE ; MAMESTRA-CONFIGURATA ; RACHIPLUSIA-OU ; GENE ; ORGANIZATION ; ALIGNMENT ; VIRUS
WOS研究方向Virology
WOS类目Virology
语种英语
WOS记录号WOS:000235930400017
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
URI标识http://www.irgrid.ac.cn/handle/1471x/2375319
专题武汉病毒研究所
通讯作者Jehle, JA
作者单位1.Agr Serv Ctr Palatinate, Lab Biotechnol Crop Protect, Dept Phytopathol, D-67435 Neustadt, Germany
2.Chinese Acad Sci, State Key Lab Virol, Wuhan 430071, Peoples R China
3.Chinese Acad Sci, Joint Lab Invertebrate Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China
推荐引用方式
GB/T 7714
Jehle, JA,Lange, M,Wang, HL,et al. Molecular identification and phylogenetic analysis of baculoviruses from lepidoptera[J]. Virology,2006,346(1):180-193.
APA Jehle, JA,Lange, M,Wang, HL,Hu, ZH,Wang, YJ,&Hauschild, W.(2006).Molecular identification and phylogenetic analysis of baculoviruses from lepidoptera.Virology,346(1),180-193.
MLA Jehle, JA,et al."Molecular identification and phylogenetic analysis of baculoviruses from lepidoptera".Virology 346.1(2006):180-193.

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来源:武汉病毒研究所

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