Heterologous expression and localization of gentisate transporter ncg12922 from corynebacterium glutamicum atcc 13032
文献类型：期刊论文
| 作者 | Xu, Y; Yan, DZ; Zhou, NY |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2006-07-28 |
| 卷号 | 346期号:2页码:555-561 |
| 关键词 | Confocal microscopy Corynebacterium glutamicum atcc 13032 Gentisate transporter Heterologous expression Ralstonia sp strain u2 |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2006.05.143 |
| 通讯作者 | Zhou, ny() |
| 英文摘要 | Ralstonia sp. strain u2 metabolizes naphthalene via gentisate (2,5-dihydroxybenzoate) to central metabolites, but it was found unable to utilize gentisate as growth substrate. a putative gentisate transporter encoded by ncg12922 from corynebacterium glutamicum atcc 13032 was functionally expressed in ralstonia sp. strain u2, converting strain u2 to a gentisate utilizer. after ncg12922 was inserted into plasmid pgfpe with green fluorescence protein gene gfp, the expressed fusion protein ncg12922-gfp could be visualized in the periphery of escherichia coli cells under confocal microscope, consistent with a cytoplasmic membrane location. in contrast, gfp was ubiquitous in the cytoplasm of e. coli cells carrying pgfpe only. gentisate 1,2-dioxygenase activity was present in the cell extract from strain u2 induced with gentisate but at a much lower level (one-fifth) than that obtained with salicylate. however, it exhibited a similar level in strain u2 containing ncg12922 induced either by salicylate or gentisate. (c) 2006 elsevier inc. all rights reserved. |
| WOS关键词 | SP STRAIN U2 ; GREEN FLUORESCENT PROTEIN ; INNER-MEMBRANE-PROTEINS ; PSEUDOMONAS-PUTIDA ; ESCHERICHIA-COLI ; GENE-CLUSTER ; NAG GENES ; NAPHTHALENE ; PERMEASE ; PATHWAY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000238631500022 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375326 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhou, NY |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Y,Yan, DZ,Zhou, NY. Heterologous expression and localization of gentisate transporter ncg12922 from corynebacterium glutamicum atcc 13032[J]. Biochemical and biophysical research communications,2006,346(2):555-561. |
| APA | Xu, Y,Yan, DZ,&Zhou, NY.(2006).Heterologous expression and localization of gentisate transporter ncg12922 from corynebacterium glutamicum atcc 13032.Biochemical and biophysical research communications,346(2),555-561. |
| MLA | Xu, Y,et al."Heterologous expression and localization of gentisate transporter ncg12922 from corynebacterium glutamicum atcc 13032".Biochemical and biophysical research communications 346.2(2006):555-561. |
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来源：武汉病毒研究所
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