Combination of scaffold and adenovirus vectors expressing bone morphogenetic protein-7 for alveolar bone regeneration at dental implant defects
文献类型：期刊论文
| 作者 | Zhang, Yufeng; Song, Jianhua; Shi, Bin; Wang, Yining; Chen, Xiaohui; Huang, Cui; Yang, Xuecao; Xu, Dongxuan; Cheng, Xiangrong; Chen, Xinwen |
| 刊名 | Biomaterials
 |
| 出版日期 | 2007-11-01 |
| 卷号 | 28期号:31页码:4635-4642 |
| 关键词 | Bone morphogenetic protein Bone Chitosan Implant Tissue engineering |
| ISSN号 | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2007.07.009 |
| 通讯作者 | Cheng, xiangrong(xiangrongcheng@hotmail.com) |
| 英文摘要 | The current rapid progression in tissue engineering and local gene delivery systems has enhanced applications of osseointegration in dental implants. in this study, porous chitosan/collagen scaffolds were prepared through a freeze-drying process, and loaded with an adenoviral vector encoding human bone morphogenetic proteins (bmp7). these scaffolds were evaluated in vitro by scanning electron microscopy (sem), and human periodontal ligament cells (hplcs) were seeded in this scaffold. we used reverse transcription-polymerase chain reaction (rt-pcr) to determine the expression levels of osteopontin and bone sialoprotein. alkaline phosphatase (alp) activity was also determined. then these scaffolds were implanted into defects on both sides of the mandible. three months later, the animals were sacrificed and non-decalcified sections were evaluated histologically. histomorphometric analyses were performed at the bone-implant interface using the image obtained by confocal laser scanning microscopy. results indicated that the scaffold containing ad-bmp7 exhibited the higher alp activity, and the expression of osteopontin and bone sialoprotein were up-regulated. after implanting in defects around implant, the bone formation in ad-bmp7 scaffolds was greater than that in other scaffolds at 4 or 8 weeks. this study demonstrated the potential of chitosan/collagen scaffold combined ad-bmp7 as a good substrate candidate in bone tissue engineering. (c) 2007 elsevier ltd. all rights reserved. |
| WOS关键词 | GUIDED TISSUE REGENERATION ; MEDIATED GENE-TRANSFER ; CELLS IN-VITRO ; PERIODONTAL-LIGAMENT ; INTRABONY DEFECTS ; STEM-CELLS ; THERAPY ; AUGMENTATION ; DIFFERENTIATION ; DELIVERY |
| WOS研究方向 | Engineering ; Materials Science |
| WOS类目 | Engineering, Biomedical ; Materials Science, Biomaterials |
| 语种 | 英语 |
| WOS记录号 | WOS:000249717500011 |
| 出版者 | ELSEVIER SCI LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375395 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Cheng, Xiangrong |
| 作者单位 | 1.Wuhan Univ, Sch Stomatol, Min Educ, Key Lab Oral Biomed Engn, Wuhan 430079, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yufeng,Song, Jianhua,Shi, Bin,et al. Combination of scaffold and adenovirus vectors expressing bone morphogenetic protein-7 for alveolar bone regeneration at dental implant defects[J]. Biomaterials,2007,28(31):4635-4642. |
| APA | Zhang, Yufeng.,Song, Jianhua.,Shi, Bin.,Wang, Yining.,Chen, Xiaohui.,...&Chen, Xinwen.(2007).Combination of scaffold and adenovirus vectors expressing bone morphogenetic protein-7 for alveolar bone regeneration at dental implant defects.Biomaterials,28(31),4635-4642. |
| MLA | Zhang, Yufeng,et al."Combination of scaffold and adenovirus vectors expressing bone morphogenetic protein-7 for alveolar bone regeneration at dental implant defects".Biomaterials 28.31(2007):4635-4642. |
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来源：武汉病毒研究所
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