中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 武汉病毒研究所
Determination and application of immunodominant regions of sars coronavirus spike and nucleocapsid proteins recognized by sera from different animal species

文献类型：期刊论文

作者Yu, Meng1; Stevens, Vicky1; Berry, Jody D.2; Crameri, Gary1; McEachern, Jennifer1; Tu, Changchun3; Shi, Zhengli4; Liang, Guodong5; Weingartl, Hana2; Cardosa, Jane
刊名Journal of immunological methods
出版日期2008-02-29
卷号331期号:1-2页码:1-12
关键词Sars Coronavirus Antibody Spike protein Nucleocapsid protein Elisa
ISSN号0022-1759
DOI10.1016/j.jim.2007.11.009
通讯作者Wang, lin-fa(linfa.wang@csiro.au)
英文摘要Knowledge of immunodominant regions in major viral antigens is important for rational design of effective vaccines and diagnostic tests. although there have been many reports of such work done for sars-cov, these were mainly focused on the immune responses of humans and mice. in this study, we aim to search for and compare immunodominant regions of the spike (s) and nucleocapsid (n) proteins which are recognized by sera from different animal species, including mouse, rat, rabbit, civet, pig and horse. twelve overlapping recombinant protein fragments were produced in escherichia coli, six each for the s and n proteins, which covered the entire coding region of the two proteins. using a membrane-strip based western blot approach, the reactivity of each antigen fragment against a panel of animal sera was determined. immunodominant regions containing linear epitopes, which reacted with sera from all the species tested, were identified for both proteins. the s3 fragment (aa 402-622) and the n4 fragment (aa 220-336) were the most immunodominant among the six s and n fragments, respectively. antibodies raised against the s3 fragment were able to block the binding of a panel of s-specific monoclonal antibodies (mab) to sars-cov in elisa, further demonstrating the immunodominance of this region. based on these findings, one-step competition elisas were established which were able to detect sars-cov antibodies from human and at least seven different animal species. considering that a large number of animal species are known to be susceptible to sars-cov, these assays will be a useful tool to trace the origin and transmission of sars-cov and to minimise the risk of animal-to-human transmission. (c) 2007 elsevier b.v. all rights reserved.
WOS关键词ACUTE-RESPIRATORY-SYNDROME ; ANGIOTENSIN-CONVERTING ENZYME-2 ; S-PROTEIN ; MONOCLONAL-ANTIBODY ; SOUTHERN CHINA ; BATS ; VIRUS ; ASSAY ; COV ; IDENTIFICATION
WOS研究方向Biochemistry & Molecular Biology ; Immunology
WOS类目Biochemical Research Methods ; Immunology
语种英语
WOS记录号WOS:000254059400001
出版者ELSEVIER SCIENCE BV
URI标识http://www.irgrid.ac.cn/handle/1471x/2375495
专题武汉病毒研究所
通讯作者Wang, Lin-Fa
作者单位1.CSIRO Livestock Ind, Australian Anim Hlth Lab, Geelong, Vic, Australia
2.CFIA, Natl Ctr Foreign Anim Dis, Winnipeg, MB, Canada
3.Acad Mil Med Sci, Inst Vet Sci, Changchun, Peoples R China
4.Chinese Acad Sci, Wuhan Inst Virol, Wuhan, Peoples R China
5.Chinese Ctr Dis Control & Prevent, Inst Viral Dis Control & Prevent, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Yu, Meng,Stevens, Vicky,Berry, Jody D.,et al. Determination and application of immunodominant regions of sars coronavirus spike and nucleocapsid proteins recognized by sera from different animal species[J]. Journal of immunological methods,2008,331(1-2):1-12.
APA Yu, Meng.,Stevens, Vicky.,Berry, Jody D..,Crameri, Gary.,McEachern, Jennifer.,...&Wang, Lin-Fa.(2008).Determination and application of immunodominant regions of sars coronavirus spike and nucleocapsid proteins recognized by sera from different animal species.Journal of immunological methods,331(1-2),1-12.
MLA Yu, Meng,et al."Determination and application of immunodominant regions of sars coronavirus spike and nucleocapsid proteins recognized by sera from different animal species".Journal of immunological methods 331.1-2(2008):1-12.

入库方式： iSwitch采集

来源：武汉病毒研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。