Neonatal neural progenitor cells and their neuronal and glial cell derivatives are fully permissive for human cytomegalovirus infection
文献类型：期刊论文
| 作者 | Luo, Min Hua1,2; Schwartz, Philip H.3; Fortunato, Elizabeth A.1 |
| 刊名 | Journal of virology
 |
| 出版日期 | 2008-10-01 |
| 卷号 | 82期号:20页码:9994-10007 |
| ISSN号 | 0022-538X |
| DOI | 10.1128/jvi.00943-08 |
| 通讯作者 | Fortunato, elizabeth a.(lfort@uidaho.edu) |
| 英文摘要 | Congenital human cytomegalovirus (hcmv) infection causes central nervous system structural abnormalities and functional disorders, affecting both astroglia and neurons with a pathogenesis that is only marginally understood. to better understand hcmv's interactions with such clinically important cell types, we utilized neural progenitor cells (npcs) derived from neonatal autopsy tissue, which can be differentiated down either glial or neuronal pathways. studies were performed using two viral isolates, towne (laboratory adapted) and tr (a clinical strain), at a multiplicity of infection of 3. npcs were fully permissive for both strains, expressing the full range of viral antigens (ags) and producing relatively large numbers of infectious virions. npcs infected with tr showed delayed development of cytopathic effects (cpe) and replication centers and shed less virus. this pattern of delay for tr infections held true for all cell types tested. differentiation of npcs was carried out for 21 days to obtain either astroglia (> 95% gfap(+)) or a 1: 5 mixed neuron/astroglia population (beta-tubulin iii+/gfap(+)). we found that both of these differentiated populations were fully permissive for hcmv infection and produced substantial numbers of infectious virions. utilizing a difference in plating efficiencies, we were able to enrich the neuron population to similar to 80% beta-tubulin iii+ cells. these beta-tubulin iii+-enriched populations remained fully permissive for infection but were very slow to develop cpe. these infected enriched neurons survived longer than either npcs or astroglia, and a small proportion were alive until at least 14 days postinfection. these surviving cells were all beta-tubulin iii+ and showed viral ag expression. surprisingly, some cells still exhibited extended processes, similar to mock-infected neurons. our findings strongly suggest neurons as reservoirs for hcmv within the developing brain. |
| WOS关键词 | MURINE CYTOMEGALOVIRUS ; PRECURSOR CELLS ; NERVOUS-SYSTEM ; AUTISTIC DISORDER ; MOUSE EMBRYO ; STEM-CELLS ; BRAIN ; DIFFERENTIATION ; REPLICATION ; VIRUS |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000260109100017 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375522 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Fortunato, Elizabeth A. |
| 作者单位 | 1.Univ Idaho, Dept Microbiol Mol Biol & Biochem, Moscow, ID 83844 USA 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Peoples R China 3.Childrens Hosp Orange Cty, Res Inst, Orange, CA 92668 USA |
| 推荐引用方式 GB/T 7714 | Luo, Min Hua,Schwartz, Philip H.,Fortunato, Elizabeth A.. Neonatal neural progenitor cells and their neuronal and glial cell derivatives are fully permissive for human cytomegalovirus infection[J]. Journal of virology,2008,82(20):9994-10007. |
| APA | Luo, Min Hua,Schwartz, Philip H.,&Fortunato, Elizabeth A..(2008).Neonatal neural progenitor cells and their neuronal and glial cell derivatives are fully permissive for human cytomegalovirus infection.Journal of virology,82(20),9994-10007. |
| MLA | Luo, Min Hua,et al."Neonatal neural progenitor cells and their neuronal and glial cell derivatives are fully permissive for human cytomegalovirus infection".Journal of virology 82.20(2008):9994-10007. |
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来源：武汉病毒研究所
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