Construction of a non-infectious sars coronavirus replicon for application in drug screening and analysis of viral protein function
文献类型：期刊论文
| 作者 | Wang, Jian-Min1; Wang, Lin-Fa2,3; Shi, Zheng-Li1 |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2008-09-12 |
| 卷号 | 374期号:1页码:138-142 |
| 关键词 | Severe acute respiratory syndrome (sars) Non-infectious replicon Drug screening Transcription Replication |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2008.06.129 |
| 通讯作者 | Shi, zheng-li(zlshi@wh.iov.cn) |
| 英文摘要 | Severe acute respiratory syndrome virus (sars-cov) was the causative agent of the sars outbreaks in 2002-2003. a safer in vitro system is desirable for conducting research on sars-cov and to screen for antiviral drugs against the virus. based on the infectious cdna clone of rsars-cov-delta e, in which the e gene has been deleted, a safe non-infectious replicon was constructed by replacing the s gene with the enhanced green fluorescent protein (egfp) gene. successful replication was achieved as evident from continuous expression of egfp detected by both fluorescence and western blot. treatment with antiviral drugs demonstrated that the replication could be significantly inhibited by 0.4 mg/ml of cysteine proteinase inhibitor e-64d, but not by ribavirin. the same replicons containing further deletion of the coding regions for non-structural proteins (nsp) 1, 2 or 16 confirmed previous observation that nsp16, but not nsp1 or nsp2, was essential for efficient viral replication or transcription. (c) 2008 elsevier inc. all rights reserved. |
| WOS关键词 | ACUTE RESPIRATORY SYNDROME ; RNA-SYNTHESIS ; INFECTIOUS CDNA ; HORSESHOE BATS ; IN-VITRO ; VIRUS ; REPLICATION ; EXPRESSION ; RELEASE ; GENOME |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000258798700027 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375529 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Shi, Zheng-Li |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.CSIRO, Australian Anim Hlth Lab, Geelong, Vic, Australia 3.Australian Biosecur Cooperat Res Ctr Emerging Inf, Geelong, Vic, Australia |
| 推荐引用方式 GB/T 7714 | Wang, Jian-Min,Wang, Lin-Fa,Shi, Zheng-Li. Construction of a non-infectious sars coronavirus replicon for application in drug screening and analysis of viral protein function[J]. Biochemical and biophysical research communications,2008,374(1):138-142. |
| APA | Wang, Jian-Min,Wang, Lin-Fa,&Shi, Zheng-Li.(2008).Construction of a non-infectious sars coronavirus replicon for application in drug screening and analysis of viral protein function.Biochemical and biophysical research communications,374(1),138-142. |
| MLA | Wang, Jian-Min,et al."Construction of a non-infectious sars coronavirus replicon for application in drug screening and analysis of viral protein function".Biochemical and biophysical research communications 374.1(2008):138-142. |
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来源：武汉病毒研究所
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