Cryptococcus neoformans directly stimulates perforin production and rearms nk cells for enhanced anticryptococcal microbicidal activity
文献类型：期刊论文
| 作者 | Marr, Kaleb J.2; Jones, Gareth J.1; Zheng, Chunfu4; Huston, Shaunna M.2; Timm-McCann, Martina1; Islam, Anowara2; Berenger, Byron M.2; Ma, Ling Ling2; Wiseman, Jeremy C. D.2; Mody, Christopher H.1,3 |
| 刊名 | Infection and immunity
 |
| 出版日期 | 2009-06-01 |
| 卷号 | 77期号:6页码:2436-2446 |
| ISSN号 | 0019-9567 |
| DOI | 10.1128/iai.01232-08 |
| 通讯作者 | Mody, christopher h.(cmody@ucalgary.ca) |
| 英文摘要 | Nk cells, in addition to possessing antitumor and antiviral activity, exhibit perforin-dependent microbicidal activity against the opportunistic pathogen cryptococcus neoformans. however, the factors controlling this response, particularly whether the pathogen itself provides an activation or rearming signal, are largely unknown. the current studies were performed to determine whether exposure to this fungus alters subsequent nk cell anticryptococcal activity. nk cells lost perforin and mobilized lysosome-associated membrane protein 1 to the cell surface following incubation with the fungus, indicating that degranulation had occurred. despite a reduced perforin content during killing, nk cells acquired an enhanced ability to kill c. neoformans, as demonstrated using auxotrophs that allowed independent assessment of the killing of two strains. de novo protein synthesis was required for optimal killing; however, there was no evidence that a soluble factor contributed to the enhanced anticryptococcal activity. exposure of nk cells to c. neoformans caused the cells to rearm, as demonstrated by increased perforin mrna levels and enhanced loss of perforin when transcription was blocked. degranulation alone was insufficient to provide the activation signal as nk cells lost anticryptococcal activity following treatment with strontium chloride. however, nk cells regained the activity upon prolonged exposure to c. neoformans, which is consistent with activation by the microbe. the enhanced cytotoxicity did not extend to tumor killing since nk cells exposed to c. neoformans failed to kill nk-sensitive tumor targets (k562 cells). these studies demonstrate that there is contact-mediated microbe-specific rearming and activation of microbicidal activity that are necessary for optimal killing of c. neoformans. |
| WOS关键词 | NATURAL-KILLER-CELLS ; CYTOTOXIC T-LYMPHOCYTES ; GRANZYME-B ; MEDIATED CYTOTOXICITY ; GENE-EXPRESSION ; PORE FORMATION ; FUNGAL TARGET ; FAS LIGAND ; IFN-GAMMA ; IN-VITRO |
| WOS研究方向 | Immunology ; Infectious Diseases |
| WOS类目 | Immunology ; Infectious Diseases |
| 语种 | 英语 |
| WOS记录号 | WOS:000266182400021 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375553 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Mody, Christopher H. |
| 作者单位 | 1.Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB T2N 4N1, Canada 2.Univ Calgary, Dept Med Sci, Calgary, AB T2N 4N1, Canada 3.Univ Calgary, Dept Internal Med, Calgary, AB T2N 4N1, Canada 4.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Marr, Kaleb J.,Jones, Gareth J.,Zheng, Chunfu,et al. Cryptococcus neoformans directly stimulates perforin production and rearms nk cells for enhanced anticryptococcal microbicidal activity[J]. Infection and immunity,2009,77(6):2436-2446. |
| APA | Marr, Kaleb J..,Jones, Gareth J..,Zheng, Chunfu.,Huston, Shaunna M..,Timm-McCann, Martina.,...&Mody, Christopher H..(2009).Cryptococcus neoformans directly stimulates perforin production and rearms nk cells for enhanced anticryptococcal microbicidal activity.Infection and immunity,77(6),2436-2446. |
| MLA | Marr, Kaleb J.,et al."Cryptococcus neoformans directly stimulates perforin production and rearms nk cells for enhanced anticryptococcal microbicidal activity".Infection and immunity 77.6(2009):2436-2446. |
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来源：武汉病毒研究所
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