Distribution, diversity, and potential mobility of extrachromosomal elements related to the bacillus anthracis pxo1 and pxo2 virulence plasmids
文献类型：期刊论文
| 作者 | Hu, Xiaomin1,2; Van der Auwera, Geraldine1; Timmery, Sophie1; Zhu, Lei3; Mahillon, Jacques1 |
| 刊名 | Applied and environmental microbiology
 |
| 出版日期 | 2009-05-15 |
| 卷号 | 75期号:10页码:3016-3028 |
| ISSN号 | 0099-2240 |
| DOI | 10.1128/aem.02709-08 |
| 通讯作者 | Mahillon, jacques(jacques.mahillon@uclouvain.be) |
| 英文摘要 | The presence of a pxo1- and/or pxo2-like plasmid(s) in clinical isolates of bacillus cereus sensu stricto and in strains of the biopesticide bacillus thuringiensis has been reported recently, and the pxo2-like plasmid pbt9727 and another pxo2-like plasmid, paw63, were found to be conjugative. in this study, a total of 1,000 b. cereus group isolates were analyzed for the presence of pxo1- and pxo2-like replicons and for the presence of pxo2-related conjugative modules. pxo1- and pxo2-like replicons were present in ca. 6.6% and 7.7% of random environmental samples, respectively, and ca. 1.54% of the strains were positive for pxo2-like transfer module genes. only the strains harboring a pxo2- like replicon also contained the corresponding transfer genes. for the strains which contained a pxo1- and/or pxo2-like replicon(s), a large plasmid(s) whose size was similar to that of pxo1-like and/or pxo2-like plasmids was also observed, but none of these isolates were found to carry the bacillus anthracis toxin or capsule virulence genes. furthermore, 17 of 22 pxo2-like plasmids containing the transfer modules were able to self-transfer and to mobilize small plasmids. no pxo1 or pxo2- like plasmid lacking the cognate transfer modules has been found to have transfer potential. in the strains possessing the putative pxo2-like conjugative apparatus, variations in the presence of the group ii introns b.th.i.1 and b.th.i.2 were observed, suggesting that there is important flexibility in the conjugation modules and their regulation. there was no consistent correlation between a pxo2-like repa dendrogram and the presence of the tra region or between a virb4 dendrogram and transfer ability. discrepancies between pxo2-like repa and virb4 dendrograms were also observed, indicating that the evolution of pxo2 is an active process. |
| WOS关键词 | GROUP-II INTRON ; THURINGIENSIS SUBSP ISRAELENSIS ; IV SECRETION SYSTEMS ; CEREUS GROUP STRAINS ; LACTOCOCCUS-LACTIS ; PCR ANALYSIS ; TOXIN GENES ; BACTERIA ; SEQUENCE ; WEIHENSTEPHANENSIS |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000265908500003 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375557 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Mahillon, Jacques |
| 作者单位 | 1.Catholic Univ Louvain, Lab Food & Environm Microbiol, B-1348 Louvain, Belgium 2.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China 3.Huazhong Agr Univ, State Key Lab Agr Microbiol, Coll Life Sci & Technol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Xiaomin,Van der Auwera, Geraldine,Timmery, Sophie,et al. Distribution, diversity, and potential mobility of extrachromosomal elements related to the bacillus anthracis pxo1 and pxo2 virulence plasmids[J]. Applied and environmental microbiology,2009,75(10):3016-3028. |
| APA | Hu, Xiaomin,Van der Auwera, Geraldine,Timmery, Sophie,Zhu, Lei,&Mahillon, Jacques.(2009).Distribution, diversity, and potential mobility of extrachromosomal elements related to the bacillus anthracis pxo1 and pxo2 virulence plasmids.Applied and environmental microbiology,75(10),3016-3028. |
| MLA | Hu, Xiaomin,et al."Distribution, diversity, and potential mobility of extrachromosomal elements related to the bacillus anthracis pxo1 and pxo2 virulence plasmids".Applied and environmental microbiology 75.10(2009):3016-3028. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。