Biochemical characterization and mutational improvement of a thermophilic esterase from sulfolobus solfataricus p2
文献类型：期刊论文
| 作者 | Shang, Yu-Shuan1,2; Zhang, Xian-En1; Wang, Xu-De1; Guo, Yong-Chao1; Zhang, Zhi-Ping1; Zhou, Ya-Feng1 |
| 刊名 | Biotechnology letters
 |
| 出版日期 | 2010-08-01 |
| 卷号 | 32期号:8页码:1151-1157 |
| 关键词 | Archaea Esterase Hyperthermophile Kinetics Sulfolobus solfataricus |
| ISSN号 | 0141-5492 |
| DOI | 10.1007/s10529-010-0274-0 |
| 通讯作者 | Zhou, ya-feng(zyf@wh.iov.cn) |
| 英文摘要 | A thermophilic esterase, ssopest, from sulfolobus solfataricus p2 was cloned and expressed in e. coli ad494 (de3). gene sequencing indicated the encoded 353 amino acids had less than 32% identity with reported esterases. the recombinant enzyme hydrolyzed p-nitrophenyl esters but not tributyrin or tricaprylin, exhibiting the highest specific activity (1.1 u/mg) with p-nitrophenyl caprylate. the enzyme was optimally active at ph 5.5 and 80a degrees c. it retained 50% activity after 1 h incubation at 80a degrees c. activity was significantly inhibited by pmsf. five ssopest mutants were generated by site-directed mutagenesis. one mutant had a higher specific activity of 2.8 u/mg at 37a degrees c and 14 u/mg at 80a degrees c than the wild-type enzyme which exhibited 0.7 u/mg at 37a degrees c and 3.8 u/mg at 80a degrees c against p-nitrophenyl butyrate. |
| WOS关键词 | CRYSTAL-STRUCTURE ; LIPASE ; CARBOXYLESTERASE ; EXTREMOPHILES ; ENZYMES ; FAMILY |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000280063500021 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375767 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhou, Ya-Feng |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shang, Yu-Shuan,Zhang, Xian-En,Wang, Xu-De,et al. Biochemical characterization and mutational improvement of a thermophilic esterase from sulfolobus solfataricus p2[J]. Biotechnology letters,2010,32(8):1151-1157. |
| APA | Shang, Yu-Shuan,Zhang, Xian-En,Wang, Xu-De,Guo, Yong-Chao,Zhang, Zhi-Ping,&Zhou, Ya-Feng.(2010).Biochemical characterization and mutational improvement of a thermophilic esterase from sulfolobus solfataricus p2.Biotechnology letters,32(8),1151-1157. |
| MLA | Shang, Yu-Shuan,et al."Biochemical characterization and mutational improvement of a thermophilic esterase from sulfolobus solfataricus p2".Biotechnology letters 32.8(2010):1151-1157. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。