Expression, purification of herpes simplex virus type 1 ul4 protein, and production and characterization of ul4 polyclonal antibody
文献类型：期刊论文
| 作者 | Pan, Weiwei1; Ren, Xiaoming2; Guo, Hong1; Ding, Qiong1; Zheng, Alan C.1 |
| 刊名 | Journal of virological methods
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 163期号:2页码:465-469 |
| 关键词 | Herpes simplex virus type 1 (hsv-1) Ul4 Protein expression Protein purification Polyclonal antibody |
| ISSN号 | 0166-0934 |
| DOI | 10.1016/j.jviromet.2009.11.015 |
| 通讯作者 | Zheng, alan c.(zheng.alan@hotmail.com) |
| 英文摘要 | The herpes simplex virus type i (hsv-1) ul4 protein is a late protein encoded by the ul4 gene. to date, the function of this protein is poorly understood. to aid further investigation of the function of this protein, the ul4 gene was cloned into the vector pet28a (+) to express his-tagged ul4 protein in escherichia coli. the recombinant fusion protein was purified from inclusion body by histidine selected nickel affinity chromatography under denaturing conditions. after refolding, the purified recombinant protein was used to produce anti-ul4 polyclonal antibody. western blot analysis demonstrated that the polyclonal sera could recognize the purified ul4 protein specifically, and in the immunofluorescence assay, the antibody was able to probe the ul4 protein with a punctate staining in hsv-1 infected cells. (c) 2009 elsevier b.v. all rights reserved. |
| WOS关键词 | INFECTED-CELL PROTEIN ; HIGH-LEVEL EXPRESSION ; COMPLETE DNA-SEQUENCE ; ESCHERICHIA-COLI ; AFFINITY TAGS ; CODON ; IDENTIFICATION ; STRATEGIES ; ARGININE ; GENES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000275074500045 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375814 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zheng, Alan C. |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Mol Virol & Viral Immunol Res Grp, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Beijing Agr Coll, Dept Vet Med, Beijing 102206, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pan, Weiwei,Ren, Xiaoming,Guo, Hong,et al. Expression, purification of herpes simplex virus type 1 ul4 protein, and production and characterization of ul4 polyclonal antibody[J]. Journal of virological methods,2010,163(2):465-469. |
| APA | Pan, Weiwei,Ren, Xiaoming,Guo, Hong,Ding, Qiong,&Zheng, Alan C..(2010).Expression, purification of herpes simplex virus type 1 ul4 protein, and production and characterization of ul4 polyclonal antibody.Journal of virological methods,163(2),465-469. |
| MLA | Pan, Weiwei,et al."Expression, purification of herpes simplex virus type 1 ul4 protein, and production and characterization of ul4 polyclonal antibody".Journal of virological methods 163.2(2010):465-469. |
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来源：武汉病毒研究所
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