Cross-protection against influenza virus infection by intranasal administration of m2-based vaccine with chitosan as an adjuvant
文献类型：期刊论文
| 作者 | Sui, Zhiwei1,4; Chen, Quanjiao1; Wu, Rui1; Zhang, Hongbo1; Zheng, Mei3; Wang, Hanzhong1; Chen, Ze1,2,3 |
| 刊名 | Archives of virology
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 155期号:4页码:535-544 |
| ISSN号 | 0304-8608 |
| DOI | 10.1007/s00705-010-0621-4 |
| 通讯作者 | Chen, ze(chenze2005@263.net) |
| 英文摘要 | Influenza vaccines based on conserved antigens could provide cross-protection against infections by multiple subtypes of influenza a virus. influenza matrix protein 2 (m2) is highly conserved in all influenza a strains. in this study, we deleted the transmembrane domain of the m2 of the avian influenza virus (aiv) a/chicken/jiangsu/7/2002 (h9n2) strain to create an m2 without a transmembrane domain, named sm2, which was efficiently expressed in escherichia coli. the sm2 protein was administered intranasally to mice in combination with chitosan adjuvant three times at an interval of 3 weeks. three weeks after the last immunization, the mice were challenged with a lethal dose (5 x ld(50)) of a/chicken/jiangsu/7/2002 (h9n2) virus, pr8 (h1n1) virus and a/chicken/henan/12/2004 (h5n1) virus. the protective immunity of the vaccine was evaluated by determining the survival rates, residual lung virus titers, body weight, and the serum antibody titers of the mice. nasal administration of 15 mu g sm2 in combination with chitosan completely protected mice against the homologous virus and protected 90 and 30% of the mice against the heterologous h1n1 and h5n1 viruses, respectively. the study indicated that the sm2 protein was a candidate antigen for a broad-spectrum influenza virus vaccine and that the adjuvant chitosan improved the efficacy of the sm2 vaccine. |
| WOS关键词 | TOXIN-B-SUBUNIT ; SYSTEMIC ANTIBODY-RESPONSES ; EXPRESSING PLASMID DNAS ; A VIRUS ; M2 PROTEIN ; BALB/C MICE ; HETEROTYPIC IMMUNITY ; MONOCLONAL-ANTIBODY ; MATRIX PROTEIN-2 ; NASAL DELIVERY |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000276125900011 |
| 出版者 | SPRINGER WIEN |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375829 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Ze |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China 3.Shanghai Inst Biol Prod, Shanghai 200052, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sui, Zhiwei,Chen, Quanjiao,Wu, Rui,et al. Cross-protection against influenza virus infection by intranasal administration of m2-based vaccine with chitosan as an adjuvant[J]. Archives of virology,2010,155(4):535-544. |
| APA | Sui, Zhiwei.,Chen, Quanjiao.,Wu, Rui.,Zhang, Hongbo.,Zheng, Mei.,...&Chen, Ze.(2010).Cross-protection against influenza virus infection by intranasal administration of m2-based vaccine with chitosan as an adjuvant.Archives of virology,155(4),535-544. |
| MLA | Sui, Zhiwei,et al."Cross-protection against influenza virus infection by intranasal administration of m2-based vaccine with chitosan as an adjuvant".Archives of virology 155.4(2010):535-544. |
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来源：武汉病毒研究所
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