Blocking peptides against hbv: pres1 protein selected from a phage display library
文献类型：期刊论文
| 作者 | Wang, Wei; Liu, Yang; Zu, Xiangyang; Jin, Rui; Xiao, Gengfu |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2011-09-09 |
| 卷号 | 412期号:4页码:633-637 |
| 关键词 | Hepatitis b virus (hbv) Pres1 Phage display Peptide Blocking |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2011.08.014 |
| 通讯作者 | Xiao, gengfu(xiaogf@wh.iov.cn) |
| 英文摘要 | The pres1 protein is present on the outermost part of the hepatitis b virus (hbv) surface and has been shown to have a pivotal function in viral infectivity and assembly. the development of reagents with high affinity and specificity for pres1 is of great significance for early diagnosis and treatment of hbv infection. a phage display library of dodecapeptide was screened for interactions with purified pres1 protein. alignment of the positive phage clones revealed a putative consensus pres1 binding motif of hxnhxmhp/r. moreover, a peptide named p7 (khmhwhppalnt) was highly enriched and occurred with a surprisingly high frequency of 72%. a thermodynamic study revealed that p7 has a higher binding affinity to pres1 than the other peptides. furthermore, p7 was able to abrogate the binding of hbv virions to the pres1 antibody, suggesting that p7 covers key functional sites on the native pres1 protein. this newly isolated peptide may, therefore, be a new therapeutic candidate for the treatment of hbv. the consensus motif could be modified to deliver imaging, diagnostic, and therapeutic agents to tissues affected by hbv. (c) 2011 elsevier inc. all rights reserved. |
| WOS关键词 | HEPATITIS-B-VIRUS ; ISOTHERMAL TITRATION CALORIMETRY ; RECEPTOR-BINDING SITE ; SURFACE PROTEIN ; PRE-S1 DOMAIN ; INFECTION ; IDENTIFICATION ; ANTIBODY ; LIGANDS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000295148500022 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375922 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Xiao, Gengfu |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Wei,Liu, Yang,Zu, Xiangyang,et al. Blocking peptides against hbv: pres1 protein selected from a phage display library[J]. Biochemical and biophysical research communications,2011,412(4):633-637. |
| APA | Wang, Wei,Liu, Yang,Zu, Xiangyang,Jin, Rui,&Xiao, Gengfu.(2011).Blocking peptides against hbv: pres1 protein selected from a phage display library.Biochemical and biophysical research communications,412(4),633-637. |
| MLA | Wang, Wei,et al."Blocking peptides against hbv: pres1 protein selected from a phage display library".Biochemical and biophysical research communications 412.4(2011):633-637. |
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来源：武汉病毒研究所
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