Up-regulation of lps-induced inos activity in dibutyryl cyclic amp differentiated rat astrocytes
文献类型：期刊论文
| 作者 | Song, W; Zhu, XZ |
| 刊名 | Acta pharmacologica sinica
 |
| 出版日期 | 1998-09-01 |
| 卷号 | 19期号:5页码:462-+ |
| 关键词 | Lipopolysaccharides Nitric-oxide synthase Cyclic amp Cell differentiation Up-regulation (physiology) Astrocytes Immunohistochemistry |
| ISSN号 | 0253-9756 |
| 通讯作者 | Zhu, xz() |
| 英文摘要 | Aim: to study the effect of dbcamp on bacterial endotoxin iss-induced nos activity. methods: microscopic changes were observed. nitrite levels were measured by fluorometric assay. nos activity was measured by citrulline assay. results: within 3-4 h after the addition of dbcamp 1 mmol . l-1 to culture medium, a morphological transformation reminiscent of in vivo, differentiation occurred. coincubation with lps and dbcamp 1 mmol . l-1 resulted in a marked increase in the nitrite production as compared with lps alone. this increase was concentration- and time-dependent with a maximal effect after 24 h treatment. nitrite production stimulated by lps is parallel to the degree of cell differentiation. after a 24-h costimulation with lps and dbcamp, l-citrulline formation assay revealed a 3-fold increase in nos activity over lps treatment alone, simultaneous incubation with l-name, completely inhibited the stimulation effect of lss/dbcamp on nitrite production, cycloheximide and dactinomycin also suppressed enhancement of nos activity stimulated by lps/ dbcamp, both in nitrite production and citrulline assay, indicating that the enhancement of nos activity was due to the expression of inducible nos (inos) gene and protein. conclusion: inflammatory signals can trigger astrocytes to express substantially different levels of inos depending on their degree of differentiation. |
| WOS关键词 | NITRIC-OXIDE SYNTHASE ; MOUSE ASTROCYTES ; PRIMARY CULTURES ; CELLS |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| WOS类目 | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000075784800017 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376043 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhu, XZ |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Dept Pharmacol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, W,Zhu, XZ. Up-regulation of lps-induced inos activity in dibutyryl cyclic amp differentiated rat astrocytes[J]. Acta pharmacologica sinica,1998,19(5):462-+. |
| APA | Song, W,&Zhu, XZ.(1998).Up-regulation of lps-induced inos activity in dibutyryl cyclic amp differentiated rat astrocytes.Acta pharmacologica sinica,19(5),462-+. |
| MLA | Song, W,et al."Up-regulation of lps-induced inos activity in dibutyryl cyclic amp differentiated rat astrocytes".Acta pharmacologica sinica 19.5(1998):462-+. |
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来源：武汉病毒研究所
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