Selection of a specific peptide from a nona-peptide library for in vitro inhibition of grass carp hemorrhage virus replication
文献类型：期刊论文
| 作者 | Wang, B; Ke, LH; Jiang, H; Li, CZ; Tien, P |
| 刊名 | Virus research
 |
| 出版日期 | 2000-04-01 |
| 卷号 | 67期号:2页码:119-125 |
| 关键词 | Grass carp hemorrhage virus Peptide library Inhibition of replication Kidney cell line |
| ISSN号 | 0168-1702 |
| 通讯作者 | Tien, p() |
| 英文摘要 | Grass carp hemorrhage virus (gchv), a member of reoviridae, causes severe hemorrhagic disease of grass carp (ctenopharyngodon idellus) in china. icosahedral virions of gchv were used as to assay the effect of specific peptides for the inhibition of gchv infectivity. a random nona-peptide library displayed on phage fuse5 was constructed, and the expressed peptides were fused onto the amino terminus of the minor coat protein iii. by biopanning, the fused peptides were bound to the biotinylated gchv. phages containing specific peptides bound to gchv were eluted and amplified in escherichia coli k91. three rounds of affinity selection enriched the pool of inhibiting peptides. sixteen clones which inhibited the replication of gchv in a grass carp kidney cell line were selected. the tcid50 of gchv was decreased over 10000 x. six clones having the strongest inhibitory effect shared the same dna sequence, with a deduced amino acid sequence of nh2-leu-trp-val-giy-gly-gly-arg-asn-ala-cooh. a synthesized nona-peptide of identical sequence exhibited similar inhibitory activity towards gchv replication in vitro. (c) 2000 elsevier science b.v. all rights reserved. |
| WOS关键词 | PHAGE DISPLAY ; LIGANDS ; ANTIBODY ; AFFINITY |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000087640200002 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376075 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Tien, P |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, B,Ke, LH,Jiang, H,et al. Selection of a specific peptide from a nona-peptide library for in vitro inhibition of grass carp hemorrhage virus replication[J]. Virus research,2000,67(2):119-125. |
| APA | Wang, B,Ke, LH,Jiang, H,Li, CZ,&Tien, P.(2000).Selection of a specific peptide from a nona-peptide library for in vitro inhibition of grass carp hemorrhage virus replication.Virus research,67(2),119-125. |
| MLA | Wang, B,et al."Selection of a specific peptide from a nona-peptide library for in vitro inhibition of grass carp hemorrhage virus replication".Virus research 67.2(2000):119-125. |
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来源：武汉病毒研究所
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