Crystal structures of yfir from pseudomonas aeruginosa in two redox states
文献类型：期刊论文
| 作者 | Yang, Xuan1; Yang, Xiu-an2,3; Xu, Min2; Zhou, Lei2,3; Fan, Zusen1; Jiang, Tao2 |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2015-05-22 |
| 卷号 | 461期号:1页码:14-20 |
| 关键词 | Biofilm Yfibnr Yfir C-di-gmp Disulfide bond Redox |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2015.03.160 |
| 通讯作者 | Jiang, tao(tjiang@ibp.ac.cn) |
| 英文摘要 | Yfibnr is a recently identified c-di-gmp regulatory system involved in bacterial biofilm formation. the periplasmic protein yfir inhibits the diguanylate cyclase activity of the inner membrane protein yfin, whereas yfib in the outer membrane can release this inhibition by sequestration of yfir. in addition, this system may respond to anoxic conditions via yfir, although the detailed mechanism is still unknown. here we report crystal structures of pseudomonas aeruginosa yfir in the absence and presence of oxidative glutathione. our structures reveal the overall folding of yfir for the first time and demonstrate that yfir exist as a dimer. comparison of the two structures in different redox states revealed a broken/formation of one disulfide bond (cys71-cys110) and local conformational change around the other one (cys145-cys152). mutagenesis studies indicated that cys145-cys152 plays an important role in maintaining the correct folding of yfir. (c) 2015 elsevier inc. all rights reserved. |
| WOS关键词 | DISULFIDE BOND FORMATION ; CYCLIC-DI-GMP ; ESCHERICHIA-COLI ; PROTEIN ; BIOFILM ; SYSTEM |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000355061000003 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376518 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Jiang, Tao |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Chinese Acad Sci Key Lab Infect & Immun, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Xuan,Yang, Xiu-an,Xu, Min,et al. Crystal structures of yfir from pseudomonas aeruginosa in two redox states[J]. Biochemical and biophysical research communications,2015,461(1):14-20. |
| APA | Yang, Xuan,Yang, Xiu-an,Xu, Min,Zhou, Lei,Fan, Zusen,&Jiang, Tao.(2015).Crystal structures of yfir from pseudomonas aeruginosa in two redox states.Biochemical and biophysical research communications,461(1),14-20. |
| MLA | Yang, Xuan,et al."Crystal structures of yfir from pseudomonas aeruginosa in two redox states".Biochemical and biophysical research communications 461.1(2015):14-20. |
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来源：中国科学院大学
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