Rapid baculovirus titration assay based on viable cell side scatter (ssc)
文献类型：期刊论文
| 作者 | Qi, Jing1; Liu, Tao1,2; Pan, Junjie1; Miao, Peng1,2; Zhang, Chun1,2 |
| 刊名 | Analytica chimica acta
 |
| 出版日期 | 2015-06-16 |
| 卷号 | 879页码:58-62 |
| 关键词 | Baculovirus expression system Virus titer Side scatter (ssc) Flow cytometer |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2015.04.007 |
| 通讯作者 | Zhang, chun(chunzhang@sibet.ac.cn) |
| 英文摘要 | The baculovirus expression system is one of the most powerful tools for the production of recombinant proteins on both laboratory and industrial scales. multiplicity of infection (moi) is the crucial parameter for efficient protein expression. to obtain an optimal moi, it is important to determine titer of virus stock before protein production. herein, we established a label-free, simple and rapid method for virus titration based on viable cell side scatter (ssc). generally, the ssc of cells infected with a series of virus dilutions was measured by a flow cytometer at 48 h post-infection, and the probability of infected cells at a given dilution was estimated. for each well with the infection probabilities between 0.20 and 0.80, the range of dilutions was chosen, and virus titer was determined with a statistical method. log-scale comparison of the results between the ssc based method and a standard plaque assay showed a good correlation (r-2 = 0.9853), suggesting the fine accuracy of this proposed method. (c) 2015 elsevier b.v. all rights reserved. |
| WOS关键词 | NUCLEAR POLYHEDROSIS-VIRUS ; POLYMERASE-CHAIN-REACTION ; INSECT CELLS ; RECOMBINANT BACULOVIRUS ; TITER DETERMINATION ; PROTEINS ; PARTICLES ; GROWTH ; LINE |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000354782600007 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376614 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, Chun |
| 作者单位 | 1.Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, CAS Key Lab Biomed Diagnost, Suzhou 215163, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Jing,Liu, Tao,Pan, Junjie,et al. Rapid baculovirus titration assay based on viable cell side scatter (ssc)[J]. Analytica chimica acta,2015,879:58-62. |
| APA | Qi, Jing,Liu, Tao,Pan, Junjie,Miao, Peng,&Zhang, Chun.(2015).Rapid baculovirus titration assay based on viable cell side scatter (ssc).Analytica chimica acta,879,58-62. |
| MLA | Qi, Jing,et al."Rapid baculovirus titration assay based on viable cell side scatter (ssc)".Analytica chimica acta 879(2015):58-62. |
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来源：中国科学院大学
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