Integration patterns of t-dna in rice genome
文献类型：期刊论文
| 作者 | Li, ST; Tian, YC; He, CZ |
| 刊名 | Progress in biochemistry and biophysics
 |
| 出版日期 | 2004-10-01 |
| 卷号 | 31期号:10页码:912-917 |
| 关键词 | Rice (oryza saliva l.) genome T-dna tag Integration pattern |
| ISSN号 | 1000-3282 |
| 通讯作者 | He, cz() |
| 英文摘要 | A t-dna tagged rice population was generated for functional genomic analysis. using thermal asymmetric interlaced pcr (tail-pcr), 159 sequences flanking the right border of t-dna were obtained. among them, 92 sequences contained the right border of t-dna and flanking rice sequences, and 78 flanking rice sequences were mapped as t-dna tags on 12 rice chromosomes. integration patterns of t-dna tags in rice genome were further analyzed using the 78 t-dna tags and 169 ones reported previously. at junctions of the right border and flanking rice sequences, 14.6% of t-dna tags (36) showed 3 similar to 74 bp of filler sequences. for the t-dna tags without filler sequences, 21.3% (45) displayed 3 similar to 5 bp of microhomology between the nicked t-dna right border and flanking rice sequences. filler sequences and microhomology revealed that both double-stranded break and single-stranded gap repair mechanisms played a critical role in the integration of t-dna into the rice genome. t-dna integration preferentially occurred in the a/t-rich regions, mainly in the 5'- and 3'-regulatory regions outside the coding regions and in introns of genes. |
| WOS关键词 | ILLEGITIMATE RECOMBINATION ; INSERTIONAL MUTAGENESIS ; ARABIDOPSIS-THALIANA ; SEQUENCE ; PLANTS ; TAGS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000224887800010 |
| 出版者 | SCIENCE CHINA PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376861 |
| 专题 | 中国科学院大学 |
| 通讯作者 | He, CZ |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Nalt Key Lab Plant Genom, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, ST,Tian, YC,He, CZ. Integration patterns of t-dna in rice genome[J]. Progress in biochemistry and biophysics,2004,31(10):912-917. |
| APA | Li, ST,Tian, YC,&He, CZ.(2004).Integration patterns of t-dna in rice genome.Progress in biochemistry and biophysics,31(10),912-917. |
| MLA | Li, ST,et al."Integration patterns of t-dna in rice genome".Progress in biochemistry and biophysics 31.10(2004):912-917. |
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来源：中国科学院大学
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