Inhibitory effect of ganglioside gd1b on k+ current in hippocampal neurons and its involvement in apoptosis suppression
文献类型：期刊论文
| 作者 | Chen, XS; Chi, SP; Liu, MN; Yang, W; Wei, TT; Qi, Z; Yang, FY |
| 刊名 | Journal of lipid research
 |
| 出版日期 | 2005-12-01 |
| 卷号 | 46期号:12页码:2580-2585 |
| 关键词 | Ion channel regulation Potassium channel Sphingolipids Lipid rafts/microdomains |
| ISSN号 | 0022-2275 |
| DOI | 10.1194/jlr.m500252-jlr200 |
| 通讯作者 | Qi, z() |
| 英文摘要 | Gangliosides are endogenous membrane components enriched in neuronal cells. they have been shown to play regulatory roles in many cellular processes. here, we show for the first time that ganglioside gd1b plays an antiapoptotic role in cultured hippocampal neurons. gd1b inhibited the voltage- dependent outward delayed rectifier current ( i-k) but not the transient outward a- type current in a dose- dependent manner, with an ic50 value of 15.2 mu m. this effect appears to be somehow specific, because gd1b, but not gm1, gm2, gm3, gd1a, gd3, or gt1b, was effective in inhibiting i k. intracellular application of staurosporine ( sts; 0.1 mu m) resulted in rapid activation of i k, which was partially reversed upon addition of the k (+) channel blocker tetraethylammonium ( tea; 5 mm) and gd1b ( 10 mu m). furthermore, gd1b ( 10 mu m) attenuated sts- induced neuronal apoptosis by nearly the same amount as 5 mm tea. in addition, gd1b suppressed the apoptosis- associated caspase 3 activation that was activated by sts. collectively, these findings suggest that gd1b plays an antiapoptotic role in cultured hippocampal neurons through its inhibitory effect on the i k and caspase activity. - chen, x., s. chi, m. liu, w. yang, t. wei, z. qi, and f. yang. inhibitory effect of ganglioside gd1b on k (+) current in hippocampal neurons and its involvement in apoptosis suppression. |
| WOS关键词 | OUTWARD POTASSIUM CURRENT ; PROGRAMMED CELL-DEATH ; LIPID RAFTS ; PLASMA-MEMBRANE ; COMPLEX GANGLIOSIDES ; NERVOUS-SYSTEM ; CHANNELS ; DIFFERENTIATION ; ACTIVATION ; ENHANCEMENT |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000233301100005 |
| 出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377240 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Qi, Z |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Brain & Cognit Sci, Inst Biophys, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Natl Lab Biomacromol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, XS,Chi, SP,Liu, MN,et al. Inhibitory effect of ganglioside gd1b on k+ current in hippocampal neurons and its involvement in apoptosis suppression[J]. Journal of lipid research,2005,46(12):2580-2585. |
| APA | Chen, XS.,Chi, SP.,Liu, MN.,Yang, W.,Wei, TT.,...&Yang, FY.(2005).Inhibitory effect of ganglioside gd1b on k+ current in hippocampal neurons and its involvement in apoptosis suppression.Journal of lipid research,46(12),2580-2585. |
| MLA | Chen, XS,et al."Inhibitory effect of ganglioside gd1b on k+ current in hippocampal neurons and its involvement in apoptosis suppression".Journal of lipid research 46.12(2005):2580-2585. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。