Cloning of a novel rice gene oslsd1 and bioinformatic analysis of lsd1-like gene family from arabidopsis and rice
文献类型:期刊论文
作者 | Wang, LJ; Tian, YC; He, CZ |
刊名 | Progress in biochemistry and biophysics
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出版日期 | 2005-03-01 |
卷号 | 32期号:3页码:268-274 |
关键词 | Lsd1-like gene Zinc finger domain Programmed cell death (pcd) Phylogenetic analysis Gene duplication |
ISSN号 | 1000-3282 |
通讯作者 | He, cz(hecz@sun.im.ac.cn) |
英文摘要 | Lsd1-like proteins are a family of plant-specific transcription factors that contain a specific class of c2c2 type zinc finger domain. two members of this family have been identified and proved to control plant programmed cell death (pcd) in arabidopsis. here, a novel rice lsd1-like gene was cloned from a cdna library and designated as oslsd1 (for oryza sativa lsd1). the cdna is 988 bp in size, containing an open reading frame of 432 bp. the predicted oslsd1 protein (143 amino acid residues) contains three internally conserved zinc finger domains. southern blot indicated that oslsd1 is a single-copy gene in rice. furthermore, the oslsd1 gene was expressed constitutively in rice root, stem and leaf. using bioinformatic tool, five and seven lsd1-like genes were identified from the databases of arabidopsis and rice, respectively. the gene structure and domain organization of these lsd1-like genes were determined. phylogenetic analysis of their coding sequences and predicted amino acid sequences suggested these genes can be classified into two classes, and a gene duplication event was identified. although there is no specific class of lsd1-like gene in arabidopsis or rice, some rice-specific zinc finger domains are found. |
WOS关键词 | PROGRAMMED CELL-DEATH ; ZINC-FINGER PROTEINS ; DRAFT SEQUENCE ; PLANT ; ANNOTATION ; THALIANA ; JAPONICA |
WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
语种 | 英语 |
WOS记录号 | WOS:000227789000013 |
出版者 | CHINESE ACAD SCIENCES, INST BIOPHYSICS |
URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377367 |
专题 | 中国科学院大学 |
通讯作者 | He, CZ |
作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Natl Key Lab Plant Genom, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, LJ,Tian, YC,He, CZ. Cloning of a novel rice gene oslsd1 and bioinformatic analysis of lsd1-like gene family from arabidopsis and rice[J]. Progress in biochemistry and biophysics,2005,32(3):268-274. |
APA | Wang, LJ,Tian, YC,&He, CZ.(2005).Cloning of a novel rice gene oslsd1 and bioinformatic analysis of lsd1-like gene family from arabidopsis and rice.Progress in biochemistry and biophysics,32(3),268-274. |
MLA | Wang, LJ,et al."Cloning of a novel rice gene oslsd1 and bioinformatic analysis of lsd1-like gene family from arabidopsis and rice".Progress in biochemistry and biophysics 32.3(2005):268-274. |
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来源:中国科学院大学
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