Palmitoylation regulates gdp/gtp exchange of g protein by affecting the gtp-binding activity of go alpha
文献类型：期刊论文
| 作者 | Cao, Y; Huang, YG |
| 刊名 | International journal of biochemistry & cell biology
 |
| 出版日期 | 2005-03-01 |
| 卷号 | 37期号:3页码:637-644 |
| 关键词 | G protein Palmitoylation Gtp-binding activity Conformation change Signal transduction |
| ISSN号 | 1357-2725 |
| DOI | 10.1016/j.biocel.2004.04.027 |
| 通讯作者 | Huang, yg() |
| 英文摘要 | The effect of palmitoylation on the gtp-binding activity and conformation of goalpha protein in hydrophobic and hydrophilic environments was studied. the binding assay was performed with an isotope labeled analog of gtp, gtp-gamma-(35) s, and its fluorescent analog, bodipy fl-gtp-gammas was used to detect conformational change in the gtp-binding domain of goa. investigation of the gtp-gamma-s-35 binding activity of goalpha shows that in a hydrophobic environment, mimicked by the presence of detergent, the apparent dissociation constant for palmitoylated goa (k-d = 25.5 x 10(-9) +/- 1.7 x 10(-9) m) increased threefold compared with that of nonpalmitoylated goa (k-d = 9.9 x 10(-9) +/- 0.8 x 10(-9) m), while in an aqueous environment without detergent there is no significant difference between palmitoylated (k-d = 50.1 x 10(-9) 5.2 x 10(-9) m) and non-palmitoylated (k-d = 65.5 x 10(-9) +/- 17.6 x 10(-9) m) goalpha. this indicates that in a membrane environment palmitoylation may weaken the gtpgammas binding ability of goalpha. fluorescent quenching studies using bodipy fl-gtpgammas as a probe showed that the conformation of the gtp-binding domain of goalpha tends to become more compact after palmitoylation. these results imply that palmitoylation may regulate the gtp/gdp exchange of goalpha by influencing the gtp-binding activity of goalpha and facilitating the on-off switch function of the g protein in g protein-coupled signal transduction. (c) 2004 elsevier ltd. all rights reserved. |
| WOS关键词 | BETA-ADRENERGIC-RECEPTOR ; MEMBRANE ASSOCIATION ; PLASMA-MEMBRANE ; SUBUNITS ; LOCALIZATION ; ACTIVATION ; G-ALPHA(Z) ; SIGNALS ; GAMMA ; PALMITATE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000226613600017 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377369 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Huang, YG |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Y,Huang, YG. Palmitoylation regulates gdp/gtp exchange of g protein by affecting the gtp-binding activity of go alpha[J]. International journal of biochemistry & cell biology,2005,37(3):637-644. |
| APA | Cao, Y,&Huang, YG.(2005).Palmitoylation regulates gdp/gtp exchange of g protein by affecting the gtp-binding activity of go alpha.International journal of biochemistry & cell biology,37(3),637-644. |
| MLA | Cao, Y,et al."Palmitoylation regulates gdp/gtp exchange of g protein by affecting the gtp-binding activity of go alpha".International journal of biochemistry & cell biology 37.3(2005):637-644. |
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来源：中国科学院大学
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