Identification and quantification of the toxic dinoflagellate gymnodinium sp with competitive enzyme-linked immunosorbent assay (celisa)
文献类型：期刊论文
| 作者 | Xin, ZY; Yu, ZG; Wang, TC; Hui, X; Gou, WL; Sun, J; Qi, HG; Li, RX |
| 刊名 | Harmful algae
 |
| 出版日期 | 2005-02-01 |
| 卷号 | 4期号:2页码:297-307 |
| 关键词 | Antibody Competitive elisa Gymnodinium sp. Quantification |
| ISSN号 | 1568-9883 |
| DOI | 10.1016/j.hal.2004.05.004 |
| 通讯作者 | Yu, zg() |
| 英文摘要 | Polyclonal antibodies were raised against gymnodinium sp. by immunizing rabbits with cells of the axenic strain. based on the species-specific antiserum, an indirect competitive enzyme-linked immunosorbent assay (celisa) was developed to identify and quantify gymnodinium sp. a standard curve was established to correlate the celisa signal to cell amount on a logit-log basis in the linear range between 24 and 6,250,000 cells, and the equation deducted was in[a/(a(0) - a)] = 4.91931. 1006 log[cell amount] (r-2 = 0.9948, n = 5). the detection limit was found to be 12 cells. the intra-assay and inter-assay coefficients of variation (cvs) were 5.8% and 9.7%, respectively. field samples collected from jiaozhou bay, china were used to assess the robustness of the method. the results showed high agreement with that of cell-counting with a light microscope. the good reproducibility and precision of the celisa implied that this new technique could be used for fast quantification of gymnodinium sp. (c) 2004 elsevier b.v. all rights reserved. |
| WOS关键词 | MONOCLONAL-ANTIBODIES ; ALEXANDRIUM-FUNDYENSE ; CHATTONELLA-ANTIQUA ; CELL-SURFACE ; IMMUNOFLUORESCENCE ; MARINA ; SAMPLES ; PHYTOPLANKTON ; DINOPHYCEAE ; SEPARATION |
| WOS研究方向 | Marine & Freshwater Biology |
| WOS类目 | Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000227169600010 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377396 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yu, ZG |
| 作者单位 | 1.Ocean Univ China, Coll Chem & Chem Engn, Qingdao 266003, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200030, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Grad Sch, Shanghai 200031, Peoples R China 4.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xin, ZY,Yu, ZG,Wang, TC,et al. Identification and quantification of the toxic dinoflagellate gymnodinium sp with competitive enzyme-linked immunosorbent assay (celisa)[J]. Harmful algae,2005,4(2):297-307. |
| APA | Xin, ZY.,Yu, ZG.,Wang, TC.,Hui, X.,Gou, WL.,...&Li, RX.(2005).Identification and quantification of the toxic dinoflagellate gymnodinium sp with competitive enzyme-linked immunosorbent assay (celisa).Harmful algae,4(2),297-307. |
| MLA | Xin, ZY,et al."Identification and quantification of the toxic dinoflagellate gymnodinium sp with competitive enzyme-linked immunosorbent assay (celisa)".Harmful algae 4.2(2005):297-307. |
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来源：中国科学院大学
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