Design and characterization of viral polypeptide inhibitors targeting newcastle disease virus fusion
文献类型：期刊论文
| 作者 | Zhu, JQ; Jiang, XL; Liu, YY; Tien, P; Gao, GF |
| 刊名 | Journal of molecular biology
 |
| 出版日期 | 2005-12-02 |
| 卷号 | 354期号:3页码:601-613 |
| 关键词 | Inhibitors design Fusion Heptad repeat (hr) Paramyxovirus Newcastle disease virus (ndv) |
| ISSN号 | 0022-2836 |
| DOI | 10.1016/j.jmb.2005.08.078 |
| 通讯作者 | Tien, p() |
| 英文摘要 | Paramyxovirus infections can be detected worldwide with some emerging zoonotic viruses and currently there are no specific therapeutic treatments or vaccines available for many of these diseases. recent studies have demonstrated that pepticies derived from the two heptad repeat regions (hr1 and hr2) of paramyxovirus fusion proteins could be used as inhibitors of virus fusion. the mechanism underlying this activity is in accordance with that of class i virus fusion proteins, of which human immunodeficiency virus (hiv) and influenza virus fusion proteins are members. for class i virus fusion proteins, the hr1 fragment binds to hr2 to form a six-helix bundle with three hr1 fragments forming the central coiled bundle surrounded by three coiled hr2 fragments in the post fusion conformational state (fusion core). it is hypothesized that the introduced exogenous hr1. or hr2 can compete against their endogenous counterparts, which results in fusion inhibition. using newcastle disease virus (ndv) as a model, we designed several protein inhibitors, denoted hr212 as well ashr121 and 5-helix, which could bind the hr1 or hr2 region of fusion protein, respectively. all the proteins were expressed and purified using a gst-fusion expression system in escherichia coli. the hr212 or gst-hr212 protein, which binds the hr1 peptide in vitro, displayed inhibitory activity against ndv-mediated cell fusion, while the hr121 and 5-helix proteins, which bind the hr2 peptide in vitro, inhibited virus fusion from the avirulent ndv strain when added before the cleavage of the fusion protein. these results showed that the designed hr212, hr121 or 5-helix protein could serve as specific antiviral agents. these data provide additional insight into the difference between the virulent and avirulent strains of ndv. (c) 2005 published by elsevier ltd. |
| WOS关键词 | HEPTAD REPEAT REGIONS ; PARAMYXOVIRUS FUSION ; MEMBRANE-FUSION ; MEASLES-VIRUS ; F-PROTEINS ; PEPTIDE INHIBITOR ; POTENT INHIBITORS ; 6-HELIX BUNDLE ; HIV-1 ENTRY ; CELL-FUSION |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000233674100010 |
| 出版者 | ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377456 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Tien, P |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Ctr Mol Virol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Chinese Ctr Dis Control & Prevent, Dept Diarrhea Viruses, Inst Viral Dis Control & Prevent China CDC, Beijing 100052, Peoples R China 4.Zhejiang Univ, Coll Life Sci, Inst Microbiol, Hangzhou 310029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, JQ,Jiang, XL,Liu, YY,et al. Design and characterization of viral polypeptide inhibitors targeting newcastle disease virus fusion[J]. Journal of molecular biology,2005,354(3):601-613. |
| APA | Zhu, JQ,Jiang, XL,Liu, YY,Tien, P,&Gao, GF.(2005).Design and characterization of viral polypeptide inhibitors targeting newcastle disease virus fusion.Journal of molecular biology,354(3),601-613. |
| MLA | Zhu, JQ,et al."Design and characterization of viral polypeptide inhibitors targeting newcastle disease virus fusion".Journal of molecular biology 354.3(2005):601-613. |
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来源：中国科学院大学
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