Ghhb1: a nonsymbiotic hemoglobin gene of cotton responsive to infection by verticillium dahliae
文献类型：期刊论文
| 作者 | Qu, ZL; Wang, HY; Xia, GX |
| 刊名 | Biochimica et biophysica acta-gene structure and expression
 |
| 出版日期 | 2005-08-15 |
| 卷号 | 1730期号:2页码:103-113 |
| 关键词 | Ghhb1 Hemoglobin Verticillium dahliae |
| ISSN号 | 0167-4781 |
| DOI | 10.1016/j.bbaexp.2005.06.009 |
| 通讯作者 | Xia, gx() |
| 英文摘要 | Verticillium wilt of cotton is a widespread and destructive disease that is caused by the fungus pathogen verticillium dahliae. although no cotton cultivar is immune to the disease, some genotypes exhibit superior wilt tolerance. to gain an insight into the molecular mechanisms responsible for wilt tolerance, we employed the method of suppression subtractive hybridization (ssh) to isolate genes whose expression is up-regulated after inoculation of the pathogen in a wilt-tolerant cotton cultivar (gossypium hirsutum cv. bd18). among the identified candidate ests, a cdna representing a nonsymbiotic hemoglobin gene (designated ghhb1) was further characterized in this study. northern blot hybridization demonstrated that ghhb1 shares similar characteristics to some other nonsymbiotic hemoglobin genes including the hypoxic stress-induced expression. sub-cellular localization analysis indicated that ghi4b1 proteins were predominantly present in the nucleus with a minor amount appearing in the cytoplasm. two novel features of ghhb1 were also identified, indicating that ghhb1 expression is activated in the cotton roots after inoculation with v dahliae and that exogenous hydrogen peroxide induces ghhb1 expression. these results suggest that the ghhb1 may play a role in the defense response of g. hirsutum against v dahliae invasion. (c) 2005 elsevier b.v. all rights reserved. |
| WOS关键词 | NON-SYMBIOTIC HEMOGLOBIN ; DISEASE-RESISTANCE RESPONSE ; NITRIC-OXIDE FUNCTIONS ; ALFALFA ROOT CULTURES ; PROGRAMMED CELL-DEATH ; GOSSYPIUM-HIRSUTUM ; REACTIVE OXYGEN ; SUSPENSION-CULTURES ; HYDROGEN-PEROXIDE ; OXIDATIVE BURST |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000231491900003 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377807 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xia, GX |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Natl Key Lab Plant Genom, Beijing 100080, Peoples R China 2.Hebei Univ, Coll Life Sci, Baoding 071002, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qu, ZL,Wang, HY,Xia, GX. Ghhb1: a nonsymbiotic hemoglobin gene of cotton responsive to infection by verticillium dahliae[J]. Biochimica et biophysica acta-gene structure and expression,2005,1730(2):103-113. |
| APA | Qu, ZL,Wang, HY,&Xia, GX.(2005).Ghhb1: a nonsymbiotic hemoglobin gene of cotton responsive to infection by verticillium dahliae.Biochimica et biophysica acta-gene structure and expression,1730(2),103-113. |
| MLA | Qu, ZL,et al."Ghhb1: a nonsymbiotic hemoglobin gene of cotton responsive to infection by verticillium dahliae".Biochimica et biophysica acta-gene structure and expression 1730.2(2005):103-113. |
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来源：中国科学院大学
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