Continuous on-line monitoring of extracellular ascorbate depletion in the rat striatum induced by global ischemia with carbon nanotube-modified glassy carbon electrode integrated into a thin-layer radial flow cell
文献类型：期刊论文
| 作者 | Zhang, MN; Liu, K; Gong, KP; Su, L; Chen, Y; Mao, LQ |
| 刊名 | Analytical chemistry
 |
| 出版日期 | 2005-10-01 |
| 卷号 | 77期号:19页码:6234-6242 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac051188d |
| 通讯作者 | Mao, lq() |
| 英文摘要 | This study describes a novel analytical system integrating in vivo microdialysis sampling with a radial thin-layer flow cell with a single-walled carbon nanotube (swnt)-modified glassy carbon electrode as working electrode for continuous and on-line monitoring of ascorbate depletion in the rat striatum induced by global ischemia. the swnts, especially those after vacuum heat treatment at 500 degrees c, are found to be able to enhance the electron-transfer kinetics of ascorbate oxidation at a low potential (ca. -50 mv) and possess a strong ability against electrode fouling. these properties essentially make it possible to determine ascorbate with a good stability and high selectivity against catecholamines and their metabolites and other electroactive species of physiological levels. while being integrated with in vivo microdialysis to assemble an on-line analytical system, the electrode is proved useful for continuous and sensitive monitoring of the basal dialysate level of ascorbate and its depletion in the rat striatum induced by global ischemia. the basal dialysate level of ascorbate is determined to be 5.0 +/- 0.5 mu m (n = 5) and a 50 +/- 10% (n = 3) depletion is recorded for the basal ascorbate after 4 h of global ischemia. |
| WOS关键词 | FREELY MOVING RATS ; SELF-ASSEMBLED MONOLAYER ; CENTRAL-NERVOUS-SYSTEM ; CEREBRAL-ISCHEMIA ; CYTOCHROME-C ; ELECTROCHEMICAL DETECTION ; SUPEROXIDE-DISMUTASE ; ACID RELEASE ; BEHAVIORAL ACTIVATION ; MERCURY-ELECTRODE |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000232443700030 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377844 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Mao, LQ |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, MN,Liu, K,Gong, KP,et al. Continuous on-line monitoring of extracellular ascorbate depletion in the rat striatum induced by global ischemia with carbon nanotube-modified glassy carbon electrode integrated into a thin-layer radial flow cell[J]. Analytical chemistry,2005,77(19):6234-6242. |
| APA | Zhang, MN,Liu, K,Gong, KP,Su, L,Chen, Y,&Mao, LQ.(2005).Continuous on-line monitoring of extracellular ascorbate depletion in the rat striatum induced by global ischemia with carbon nanotube-modified glassy carbon electrode integrated into a thin-layer radial flow cell.Analytical chemistry,77(19),6234-6242. |
| MLA | Zhang, MN,et al."Continuous on-line monitoring of extracellular ascorbate depletion in the rat striatum induced by global ischemia with carbon nanotube-modified glassy carbon electrode integrated into a thin-layer radial flow cell".Analytical chemistry 77.19(2005):6234-6242. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。