Phosphatidic acid osmotically destabilizes lysosomes through increased permeability to k+ and h+
文献类型：期刊论文
| 作者 | Yi, Y. P.; Wang, X.; Zhang, G.; Fu, T. -S.; Zhang, G. -J. |
| 刊名 | General physiology and biophysics
 |
| 出版日期 | 2006-06-01 |
| 卷号 | 25期号:2页码:149-160 |
| 关键词 | Lysosome Phosphatidic acid Potassium ion Proton Osmotic sensitivity |
| ISSN号 | 0231-5882 |
| 通讯作者 | Zhang, g. -j.(zhanggj@sun5.ibp.ac.cn) |
| 英文摘要 | Lysosomal destabilization is a critical event not only for the organelle but also for living cells. however, what factors can affect lysosomal stability is not fully studied. in this work, the effects of phosphatidic acid (pa) on the lysosomal integrity were investigated. through the measurements of lysosomal beta-hexosaminidase free activity, intralysosomal ph, leakage of lysosomal protons and lysosomal latency loss in hypotonic sucrose medium, we established that pa could increase the lysosomal permeability to k+ and h+, and enhance the lysosomal osmotic sensitivity. treatment of lysosomes with pa promoted entry of k+ into the organelle via k+/h+ exchange, which could produce osmotic stresses and osmotically destabilize the lysosomes. in addition, pa-induced increase in the lysosomal osmotic sensitivity caused the lysosomes to become more liable to destabilization in osmotic shocks. the results suggest that pa may play a role in the lysosomal destabilization. |
| WOS关键词 | PHOSPHOLIPASE-D ; LYSOPHOSPHATIDIC ACID ; ENZYME RELEASE ; PROTON PUMP ; RAT-LIVER ; MEMBRANE ; TRANSLOCATION ; PATHOGENESIS ; ENHANCEMENT ; INVOLVEMENT |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Physiology |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics ; Physiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000240213000004 |
| 出版者 | GENERAL PHYSIOL AND BIOPHYSICS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378528 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, G. -J. |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China 2.Hunan Agr Univ, Changsha, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China 4.Univ Sci & Technol Beijing, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yi, Y. P.,Wang, X.,Zhang, G.,et al. Phosphatidic acid osmotically destabilizes lysosomes through increased permeability to k+ and h+[J]. General physiology and biophysics,2006,25(2):149-160. |
| APA | Yi, Y. P.,Wang, X.,Zhang, G.,Fu, T. -S.,&Zhang, G. -J..(2006).Phosphatidic acid osmotically destabilizes lysosomes through increased permeability to k+ and h+.General physiology and biophysics,25(2),149-160. |
| MLA | Yi, Y. P.,et al."Phosphatidic acid osmotically destabilizes lysosomes through increased permeability to k+ and h+".General physiology and biophysics 25.2(2006):149-160. |
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来源：中国科学院大学
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