Construction and characterization of a transformation-competent artificial chromosome (tac) library of zizania latifolia (griseb.)
文献类型：期刊论文
| 作者 | Kong, Fan Na; Jiang, Shao Mei; Shi, Li Xue; Song, Cheng Li; Jin, De Min; Jiang, Shao Lin; Weng, Manli; Wang, Bin |
| 刊名 | Plant molecular biology reporter
 |
| 出版日期 | 2006-06-01 |
| 卷号 | 24期号:2页码:219-227 |
| 关键词 | Dihydrodipicolinate synthase (dhps) Genome library Z. latifolia (griseb). Turcz. ex stapf Tac Transformation-competent artificial chromosome |
| ISSN号 | 0735-9640 |
| 通讯作者 | Jiang, shao mei(shmjiang@yahoo.com.cn) |
| 英文摘要 | The tribe oryzeae consists of 12 genera and 71 species with a world distribution. zizania latifolia (griseb.) turcz. ex stapf is included in this tribe and possesses numerous traits valuable for rice breeding, such as disease and insect resistance, cold and flood tolerance, and high grain quality. the genetics and breeding of z. latifolia are still in their infancy. to facilitate genomic studies of zizania, a genomic dna library was constructed using a transformation-competent artificial chromosome (tac) vector system. the tac library contains 91, 584 tac clones with an average insert size of approximately 45 kb, covering six haploid zizania genome equivalents. very low signals after hybridization with chloroplast and mitochondrial genes indicate that the tac library is predominantly composed of nuclear dna. the tac clones were stable in e. coli for 100 generations. clones containing the dihydrodipicolinate synthase (dhps) gene were screened by pooled pcr. the positive clones can be used for z. latifolia dhps gene cloning and functional analysis. the library will be useful in studies of genome structure, gene cloning and evolution of rice. |
| WOS关键词 | RICE ORYZA-SATIVA ; DIHYDRODIPICOLINATE SYNTHASE ; COMPARATIVE MAP ; PALUSTRIS ; CLONING ; VECTOR ; WHEAT ; DNA ; L. ; PURIFICATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS类目 | Biochemical Research Methods ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000242633000006 |
| 出版者 | INT SOC PLANT MOLECULAR BIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378667 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Jiang, Shao Mei |
| 作者单位 | 1.Jiangxi Normal Univ, Coll Life Sci, Nanchang 100093, Peoples R China 2.Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.Shandong Normal Univ, Coll Hort, Tai An 271018, Peoples R China 5.Jiangxi Agr Univ, Nanchang 330022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Kong, Fan Na,Jiang, Shao Mei,Shi, Li Xue,et al. Construction and characterization of a transformation-competent artificial chromosome (tac) library of zizania latifolia (griseb.)[J]. Plant molecular biology reporter,2006,24(2):219-227. |
| APA | Kong, Fan Na.,Jiang, Shao Mei.,Shi, Li Xue.,Song, Cheng Li.,Jin, De Min.,...&Wang, Bin.(2006).Construction and characterization of a transformation-competent artificial chromosome (tac) library of zizania latifolia (griseb.).Plant molecular biology reporter,24(2),219-227. |
| MLA | Kong, Fan Na,et al."Construction and characterization of a transformation-competent artificial chromosome (tac) library of zizania latifolia (griseb.)".Plant molecular biology reporter 24.2(2006):219-227. |
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来源：中国科学院大学
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