Fusion of chemotactic peptide to a single-chain bi-specific antibody (scbsab) potentiates its cytotoxicity to target tumour cells
文献类型：期刊论文
| 作者 | Song, Jingzhen; Wang, Xiangbin; Lei, Chun; Piao, Jinhua; Yin, Changcheng; Zhang, Zhong; Lin, Qing; Huang, Hualiang |
| 刊名 | Biotechnology and applied biochemistry
 |
| 出版日期 | 2006-11-01 |
| 卷号 | 45页码:147-154 |
| 关键词 | Bi-specific antibody Chemotactic peptide Cytotoxicity Ovarian carcinoma Peripheral-blood lymphocyte Tumour-specific cytolysis |
| ISSN号 | 0885-4513 |
| DOI | 10.1042/ba20060060 |
| 通讯作者 | Huang, hualiang(hlhuang@genetics.ac.cn) |
| 英文摘要 | Anti-tumour bsab (bi-specific antibody) has been proved very effective in killing tumour cells both in vitro and in vivo. in order to enhance its ability to recruit and activate t-lymphocytes and then improve tumour-specific cytolysis, an anti-ovarian carcinoma/cd3 bsab, bhl-i, was fused to n-terminal 18 peptide of ccl21 (cc chemokine ligand 21) to produce a new chemotactic bsab, named 18tbhl. it was expressed in soluble form in the cytoplasm of escherichia coli and purified with deae anion-exchange chromatography and immobilized-metal-ion affinity chromatography. the chemotactic ability of 18tbhl to pbls (peripheral-blood lymphocytes) was detected by boyden chamber chemotaxis assay. the specific ability to bind to ovarian carcinoma cells, skov3, and pbls was tested by elisa and flow cytometry. just as expected, the enhanced tumour-specific cytolysis of 18tbhl was validated by mtt [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2h-tetrazolium bromide] method and flow cytometry. the results indicated that fusion of chemotactic peptide to bsab potentiated its cytotoxicity to tumour cells in vitro. it suggests that 18tbhl may be a promising candidate agent in cancer immunotherapy. |
| WOS关键词 | LYMPHOID-TISSUE CHEMOKINE ; IMMUNODEFICIENCY-VIRUS TYPE-1 ; N-TERMINAL PEPTIDES ; BISPECIFIC ANTIBODIES ; CC-CHEMOKINE ; FACTOR-I ; RECEPTOR-BINDING ; DENDRITIC CELLS ; T-LYMPHOCYTES ; PROTEIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000241975100005 |
| 出版者 | PORTLAND PRESS LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379421 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Huang, Hualiang |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Grad Univ, Chinese Acad Sci, Beijing 100049, Peoples R China 3.Beijing ABT Genet Engn Technol Co Ltd, Beijing 102206, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Jingzhen,Wang, Xiangbin,Lei, Chun,et al. Fusion of chemotactic peptide to a single-chain bi-specific antibody (scbsab) potentiates its cytotoxicity to target tumour cells[J]. Biotechnology and applied biochemistry,2006,45:147-154. |
| APA | Song, Jingzhen.,Wang, Xiangbin.,Lei, Chun.,Piao, Jinhua.,Yin, Changcheng.,...&Huang, Hualiang.(2006).Fusion of chemotactic peptide to a single-chain bi-specific antibody (scbsab) potentiates its cytotoxicity to target tumour cells.Biotechnology and applied biochemistry,45,147-154. |
| MLA | Song, Jingzhen,et al."Fusion of chemotactic peptide to a single-chain bi-specific antibody (scbsab) potentiates its cytotoxicity to target tumour cells".Biotechnology and applied biochemistry 45(2006):147-154. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。