Complementation analyses of sinorhizobium meliloti nifa mutant with different originated nifa genes
文献类型：期刊论文
| 作者 | Yao Zhenhua; Tian Zhexian; Dai Xiaomi; Becker, Anke; Li Jian; Yan Haiqin; Xiao Yan; Zhu Jiabi; Yu Guanqiao; Rueverg, Silvia |
| 刊名 | Chinese science bulletin
 |
| 出版日期 | 2006-11-01 |
| 卷号 | 51期号:22页码:2748-2754 |
| 关键词 | Sinorhizobium meliloti Nifa Gene expression Microarray |
| ISSN号 | 1001-6538 |
| DOI | 10.1007/s11434-006-2203-0 |
| 通讯作者 | Zou huasong(hszhou@sibs.ac.cn) |
| 英文摘要 | A previous work inferred that the nifa gene of enterobacter cloacae did not restore the symbiotic phenotype of sinorhizobium meliloti nifa mutant. in the present study, two nifa genes of bradyrhizobiurn japonicum and mesorhizobium huakuii also did not restore the symbiotic phenotype of s. meliloti nifa mutant. in whole genomic microarray experiments, 238 genes were found to be differentially expressed after s. meliloti nifa had been constitutively expressed in its nifa mutant. in contrast, only 20, 7 and 9 genes changed their transcriptional levels when expressing b. japonium, m. huakuii and enterobacter cloacae nifa genes in sm nifa mutant separately. these genes were classified into severai functional groups including house keeping, energy and central intermediary metabolism, transport systems and symbiosis. interestingly, the genes that of nifh operons showed high expression levels in the presence of either b. japonium or m. huakuii nifa, which was confirmed by subsequent lacz fusion experiments. |
| WOS关键词 | RHIZOBIUM-LEGUME SYMBIOSIS ; NITROGEN-FIXATION ; NODULE ; EXPRESSION |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000242609200012 |
| 出版者 | SCIENCE CHINA PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379760 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zou Huasong |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 2.Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China 3.Univ Bielefeld, Lehrstuhl Genet, D-33615 Bielefeld, Germany 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao Zhenhua,Tian Zhexian,Dai Xiaomi,et al. Complementation analyses of sinorhizobium meliloti nifa mutant with different originated nifa genes[J]. Chinese science bulletin,2006,51(22):2748-2754. |
| APA | Yao Zhenhua.,Tian Zhexian.,Dai Xiaomi.,Becker, Anke.,Li Jian.,...&Zou Huasong.(2006).Complementation analyses of sinorhizobium meliloti nifa mutant with different originated nifa genes.Chinese science bulletin,51(22),2748-2754. |
| MLA | Yao Zhenhua,et al."Complementation analyses of sinorhizobium meliloti nifa mutant with different originated nifa genes".Chinese science bulletin 51.22(2006):2748-2754. |
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来源：中国科学院大学
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