Pig11 protein binds to dna in sequence-independent manner in vitro
文献类型：期刊论文
| 作者 | Xiong, Xiu-Fang; Li, Hui; Cao, En-Hua |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2007-06-22 |
| 卷号 | 358期号:1页码:29-34 |
| 关键词 | Pig11 protein Dna binding Sequence-independent Gel retardation assay Atomic force microscopy |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2007.04.048 |
| 通讯作者 | Cao, en-hua(caoeh@sun5.ibp.ac.cn) |
| 英文摘要 | Pig11 (p53-induced protein 11), one of early transcriptional targets of tumor suppressor p53, was up-regulated in the induction of apoptosis or cell growth inhibition by multiple chemopreventive agents. however, its biological role remains unclear. here, we expressed his(6)-tagged pig11 protein in escherichia coli and demonstrated the recombinant his(6)-tagged pig11 protein could bind to supercoiled and relaxed closed circular plasmid dna or linear dna with different length using gel retardation assays in vitro. the interaction between dna and pig11 protein was sequence-independent and related to charge effect. the reducing thiol group in pig 11 protein was involved in the binding activity of pig11 to dna. furthermore, the images of atomic force microscopy directly confirmed the binding of dna and pig11 protein and showed the pig11-dna complex formed a beads-on-a-string appearance in which pig11 protein associated with dna as polymer. these findings suggest that pig11 protein may play an important role by interaction with other biological molecules in the regulation of apoptosis and provided us a novel angel of view to explore the possible function of pig11 in vivo. (c) 2007 elsevier inc. all rights reserved. |
| WOS关键词 | APOPTOSIS-INDUCING FACTOR ; ATOMIC-FORCE MICROSCOPY ; EXPRESSION PROFILES ; ARSENIC TRIOXIDE ; GENE ; CELLS ; CANCER ; MODEL ; P53 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000246790800006 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380118 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Cao, En-Hua |
| 作者单位 | Chinese Acad Sci, Grad Sch, Inst Biophys, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiong, Xiu-Fang,Li, Hui,Cao, En-Hua. Pig11 protein binds to dna in sequence-independent manner in vitro[J]. Biochemical and biophysical research communications,2007,358(1):29-34. |
| APA | Xiong, Xiu-Fang,Li, Hui,&Cao, En-Hua.(2007).Pig11 protein binds to dna in sequence-independent manner in vitro.Biochemical and biophysical research communications,358(1),29-34. |
| MLA | Xiong, Xiu-Fang,et al."Pig11 protein binds to dna in sequence-independent manner in vitro".Biochemical and biophysical research communications 358.1(2007):29-34. |
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来源：中国科学院大学
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