中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Pig11 protein binds to dna in sequence-independent manner in vitro

文献类型:期刊论文

作者Xiong, Xiu-Fang; Li, Hui; Cao, En-Hua
刊名Biochemical and biophysical research communications
出版日期2007-06-22
卷号358期号:1页码:29-34
关键词Pig11 protein Dna binding Sequence-independent Gel retardation assay Atomic force microscopy
ISSN号0006-291X
DOI10.1016/j.bbrc.2007.04.048
通讯作者Cao, en-hua(caoeh@sun5.ibp.ac.cn)
英文摘要Pig11 (p53-induced protein 11), one of early transcriptional targets of tumor suppressor p53, was up-regulated in the induction of apoptosis or cell growth inhibition by multiple chemopreventive agents. however, its biological role remains unclear. here, we expressed his(6)-tagged pig11 protein in escherichia coli and demonstrated the recombinant his(6)-tagged pig11 protein could bind to supercoiled and relaxed closed circular plasmid dna or linear dna with different length using gel retardation assays in vitro. the interaction between dna and pig11 protein was sequence-independent and related to charge effect. the reducing thiol group in pig 11 protein was involved in the binding activity of pig11 to dna. furthermore, the images of atomic force microscopy directly confirmed the binding of dna and pig11 protein and showed the pig11-dna complex formed a beads-on-a-string appearance in which pig11 protein associated with dna as polymer. these findings suggest that pig11 protein may play an important role by interaction with other biological molecules in the regulation of apoptosis and provided us a novel angel of view to explore the possible function of pig11 in vivo. (c) 2007 elsevier inc. all rights reserved.
WOS关键词APOPTOSIS-INDUCING FACTOR ; ATOMIC-FORCE MICROSCOPY ; EXPRESSION PROFILES ; ARSENIC TRIOXIDE ; GENE ; CELLS ; CANCER ; MODEL ; P53
WOS研究方向Biochemistry & Molecular Biology ; Biophysics
WOS类目Biochemistry & Molecular Biology ; Biophysics
语种英语
WOS记录号WOS:000246790800006
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
URI标识http://www.irgrid.ac.cn/handle/1471x/2380118
专题中国科学院大学
通讯作者Cao, En-Hua
作者单位Chinese Acad Sci, Grad Sch, Inst Biophys, Beijing 100101, Peoples R China
推荐引用方式
GB/T 7714
Xiong, Xiu-Fang,Li, Hui,Cao, En-Hua. Pig11 protein binds to dna in sequence-independent manner in vitro[J]. Biochemical and biophysical research communications,2007,358(1):29-34.
APA Xiong, Xiu-Fang,Li, Hui,&Cao, En-Hua.(2007).Pig11 protein binds to dna in sequence-independent manner in vitro.Biochemical and biophysical research communications,358(1),29-34.
MLA Xiong, Xiu-Fang,et al."Pig11 protein binds to dna in sequence-independent manner in vitro".Biochemical and biophysical research communications 358.1(2007):29-34.

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来源:中国科学院大学

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