Existence and characterization of allelic variants of sao, a newly identified surface protein from streptococcus suis
文献类型：期刊论文
| 作者 | Feng, Youjun; Zheng, Feng; Pan, Xiuzhen; Sun, Wen; Wang, Changjun; Dong, Yaqing; Ju, Ai-ping; Ge, Junchao; Liu, Di; Liu, Cuihua |
| 刊名 | Fems microbiology letters
 |
| 出版日期 | 2007-10-01 |
| 卷号 | 275期号:1页码:80-88 |
| 关键词 | Streptococcus suis Sao protein Allelic variants Antibody Elisa |
| ISSN号 | 0378-1097 |
| DOI | 10.1111/j.1574-6968.2007.00859.x |
| 通讯作者 | Gao, george f.(gaof@im.ac.cn) |
| 英文摘要 | Surface antigen one (sao) is a newly identified protein from the major zoonotic pathogen, streptococcus suis. in search of functional proteins related to the pathogenesis of chinese s. suis 2 (ss2), unexpectedly, a variant of sao protein was obtained. to test its prevalence in s. suis, pcr assay was adopted to address the coding genes systematically. it was found that there are three allelic variants of sao gene, namely sao-s, sao-m, and sao-l based on the different lengths of the genes (similar to 1.5, similar to 1.7, and similar to 2.0 kb, respectively). these differences were determined to be caused by heterogeneity within the number of c-terminal repeat sequences (r), which had been seen as a pathogenicity-related domain in the plant pathogen, xanthomonas oryzae. two variants (sao-m and sao-l) were only found in ss2. all three variant proteins were prepared in vitro and their biochemical and biophysical properties were characterized. a soluble form of sao-m protein was then used as a capture antigen to develop an enzyme-linked immunosorbent assay method to detect antibodies against ss2 in convalescent pig sera. taken together, the results exhibit the properties of sao proteins and provide an efficient sao-m-based method for monitoring ss2 infection. |
| WOS关键词 | GRAM-POSITIVE BACTERIA ; BINDING PROTEIN ; FUSION CORE ; SEROTYPE-2 ; GENE ; PATHOGENESIS ; VIRULENCE ; SUILYSIN ; CLONING ; SUPERANTIGENS |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000249428700011 |
| 出版者 | BLACKWELL PUBLISHING |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2382022 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Gao, George F. |
| 作者单位 | 1.Chinese Acad Sci, Ctr Mol Immunol, Inst Microbiol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Res Inst Med Nanjing Command, Dept Epidemiol, Nanjing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Youjun,Zheng, Feng,Pan, Xiuzhen,et al. Existence and characterization of allelic variants of sao, a newly identified surface protein from streptococcus suis[J]. Fems microbiology letters,2007,275(1):80-88. |
| APA | Feng, Youjun.,Zheng, Feng.,Pan, Xiuzhen.,Sun, Wen.,Wang, Changjun.,...&Gao, George F..(2007).Existence and characterization of allelic variants of sao, a newly identified surface protein from streptococcus suis.Fems microbiology letters,275(1),80-88. |
| MLA | Feng, Youjun,et al."Existence and characterization of allelic variants of sao, a newly identified surface protein from streptococcus suis".Fems microbiology letters 275.1(2007):80-88. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。